Biodiversity and Palaeobiology Group, National Fungal Culture Collection of India (NFCCI), MACS-Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, India.
Savitribai Phule Pune University, Ganeshkhind, Pune, 411007, India.
Arch Microbiol. 2022 Apr 2;204(4):237. doi: 10.1007/s00203-022-02841-w.
Three strains, SMT1.3, SMT1.10, and SMT2.2, representing a novel asexual ascomycetous yeast species, were isolated from the gut of a termite Odontotermes horni in Maharashtra, India. Phylogenetic analyses of the LSU, ITS, and SSU sequences revealed that they belonged to the genus Nakazawaea, with N. siamensis as the closest relative. The new species differed from the type strain of N. siamensis (DMKU-RK467) by 11 substitutions in the D1/D2 region of the large subunit (LSU) rRNA gene and by 8 substitutions and one gap in the small subunit (SSU) rRNA gene. Notable biochemical and physiological differences were also observed between N. siamensis and the new species. Hence, the species Nakazawaea odontotermitis f.a., sp. nov. is proposed. The type strain is SMT1.3 (MTCC 13,105 = NFCCI 5011 = PYCC 9153). GenBank accession numbers of the LSU, ITS and SSU sequences of Nakazawaea odontotermitis f.a., sp. nov. are MZ234240, MZ234239, and OK384663. The MycoBank number is MB 841926.
从印度马哈拉施特拉邦的一种白蚁 Odontotermes horni 的肠道中分离到三株菌株，SMT1.3、SMT1.10 和 SMT2.2，代表一种新型的无性子囊菌酵母种。LSU、ITS 和 SSU 序列的系统发育分析表明，它们属于 Nakazawaea 属，与 N. siamensis 关系最密切。新物种与 N. siamensis（DMKU-RK467）的模式株在大亚基（LSU）rRNA 基因的 D1/D2 区有 11 个取代，在小亚基（SSU）rRNA 基因有 8 个取代和 1 个缺口。还观察到 Nakazawaea 和新物种之间在生化和生理上有显著差异。因此，提出了 Nakazawaea odontotermitis f.a.，sp. nov. 这个新种。模式株为 SMT1.3（MTCC 13,105=NFCCI 5011=PYCC 9153）。Nakazawaea odontotermitis f.a.，sp. nov. 的 LSU、ITS 和 SSU 序列的 GenBank 登录号分别为 MZ234240、MZ234239 和 OK384663。MycoBank 编号为 MB 841926。
