School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, People's Republic of China.
Shanghai Energy New Material Technology Co., Ltd, , Shanghai, 201399, People's Republic of China.
Appl Biochem Biotechnol. 2022 Aug;194(8):3419-3434. doi: 10.1007/s12010-022-03892-x. Epub 2022 Apr 2.
Peptide profiling based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is of particular interest as it can provide physiologically and pathologically related information of the bio-samples. Due to the complexity of real biological samples, MALDI-TOF MS-based peptide mapping methods rely strongly on particular enrichment methods to improve the signal intensity. This paper introduces third-generation dendrimer-modified SBA-15 with the surface functionalization of amino and carboxyl group, respectively (denoted as SBA-15/G3-NH and SBA-15/G3-COOH), for the efficient capture of low-abundance peptides. The enrichment ability of the nanocomposites was evaluated by standard peptides digests and real biological samples. The synthesized nanocomposites incorporated the benefit of dendrimers and mesoporous silica nanomaterial SBA-15, showing enhanced peptide enrichment ability. Therefore, this work may provide a new class of nanomaterials for peptide mapping from biological samples.
基于基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）的肽谱分析特别有趣，因为它可以提供与生物样本相关的生理和病理信息。由于实际生物样本的复杂性，基于 MALDI-TOF MS 的肽图谱方法强烈依赖于特定的富集方法来提高信号强度。本文介绍了第三代树状大分子修饰的 SBA-15，分别对其表面进行氨基和羧基功能化（分别表示为 SBA-15/G3-NH 和 SBA-15/G3-COOH），用于有效捕获低丰度肽。通过标准肽消化物和真实生物样品评估了纳米复合材料的富集能力。合成的纳米复合材料结合了树状大分子和介孔硅纳米材料 SBA-15 的优点，表现出增强的肽富集能力。因此，这项工作可能为从生物样品中进行肽图谱分析提供了一类新的纳米材料。
