Fu Xin, Li Sheng, Jia Minzhi, Xu Bo, Yang Lele, Ma Ruimiao, Cheng Hong, Yang Wenjun, Hu Ping
Spine Center, Department of Pediatric Orthopedics, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, 200092, China.
State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, 200031, China.
Cell Regen. 2022 Apr 3;11(1):13. doi: 10.1186/s13619-022-00114-x.
Long non-coding (lnc) RNA plays important roles in many cellular processes. The function of the vast majority of lncRNAs remains unknown. Here we identified that lncRNA-1700113A16RIK existed in skeletal muscle stem cells (MuSCs) and was significantly elevated during MuSC differentiation. Knockdown of 1700113A16RIK inhibits the differentiation of muscle stem cells. In contrast, overexpression of 1700113A16RIK promotes the differentiation of muscle stem cells. Further study shows the muscle specific transcription factor Myogenin (MyoG) positively regulates the expression of 1700113A16RIK by binding to the promoter region of 1700113A16RIK. Mechanistically, 1700113A16RIK may regulate the expression of myogenic genes by directly binding to 3'UTR of an important myogenic transcription factor MEF2D, which in turn promotes the translation of MEF2D. Taken together, our results defined 1700113A16RIK as a positive regulator of MuSC differentiation and elucidated a mechanism as to how 1700113A16RIK regulated MuSC differentiation.
长链非编码（lnc）RNA在许多细胞过程中发挥着重要作用。绝大多数lncRNA的功能仍然未知。在这里，我们发现lncRNA - 1700113A16RIK存在于骨骼肌干细胞（MuSCs）中，并且在MuSC分化过程中显著升高。敲低1700113A16RIK会抑制肌肉干细胞的分化。相反，过表达1700113A16RIK会促进肌肉干细胞的分化。进一步的研究表明，肌肉特异性转录因子肌细胞生成素（MyoG）通过与1700113A16RIK的启动子区域结合来正向调节1700113A16RIK的表达。从机制上讲，1700113A16RIK可能通过直接结合重要的肌源性转录因子MEF2D的3'UTR来调节肌源性基因的表达，进而促进MEF2D的翻译。综上所述，我们的结果将1700113A16RIK定义为MuSC分化的正向调节因子，并阐明了1700113A16RIK调节MuSC分化的机制。
