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一种感染卡西棒孢菌的新型威克病毒的分子特征分析

Molecular characterization of a novel victorivirus infecting Corynespora cassiicola.

作者信息

Liu Mingming, Ni Yunxia, Zhao Hui, Liu Xintao, Jia Min, Liu Hongyan, Tian Baoming

机构信息

Institute of Plant Protection, Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Key Laboratory of Crop Pest Control, Henan Academy of Agricultural Sciences, Postgraduate T&R Base of Zhengzhou University, 450002, Zhengzhou, Henan, China.

School of Agricultural Sciences, Zhengzhou University, 450001, Zhengzhou, Henan, China.

出版信息

Arch Virol. 2022 May;167(5):1365-1368. doi: 10.1007/s00705-022-05394-x. Epub 2022 Apr 3.

Abstract

A novel victorivirus was detected in an isolate of Corynespora cassiicola strain 20180909-03 and was named "Corynespora cassiicola victorivirus 1" (CcVV1). The complete genome sequence of this virus is 5140 bp in length and contains 57% GC with two large open reading frames (ORFs) overlapping at the tetranucleotide AUGA. The ORFs were predicted to encode a coat protein (CP) and an RNA-dependent RNA polymerase (RdRp), respectively, which are conserved in dsRNA fungal viruses of the family Totiviridae. Comparison and phylogenetic analysis of the deduced amino acid sequences of RdRp and CP showed that CcVV1 is a new member of the genus Victorivirus. This is the first report of a genomic sequence of a victorivirus infecting Corynespora cassiicola.

摘要

在尖孢炭疽菌菌株20180909 - 03的一个分离物中检测到一种新型维克托病毒,并将其命名为“尖孢炭疽菌维克托病毒1”(CcVV1)。该病毒的完整基因组序列长度为5140 bp,GC含量为57%,有两个大的开放阅读框(ORF)在四核苷酸AUGA处重叠。预测这两个ORF分别编码一个衣壳蛋白(CP)和一个RNA依赖的RNA聚合酶(RdRp),它们在双链RNA真菌病毒的双分病毒科中是保守的。对RdRp和CP推导的氨基酸序列进行比较和系统发育分析表明,CcVV1是维克托病毒属的一个新成员。这是关于感染尖孢炭疽菌的维克托病毒基因组序列的首次报道。

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