Vinores S A, Herman M M, Rubinstein L J
Histopathology. 1986 Sep;10(9):891-908. doi: 10.1111/j.1365-2559.1986.tb02588.x.
A series of primary and metastatic human brain tumours was evaluated immunocytochemically for the electron microscopic localization of neuron-specific enolase (NSE). All contained cells which, regardless of the cell type, demonstrated an irregular distribution of NSE in their cytoplasm and on membranes. This was in contrast to the staining pattern in normal central nervous system (CNS) cells which, as previously reported (Vinores et al. 1984b), show only diffuse cytoplasmic staining usually not associated with membranes. In the tumours, the interior of nuclei and the cristae and matrices of mitochondria were consistently negative, as in normal CNS cells. Except in one low-grade fibrillary astrocytoma, the cytoplasmic filaments in neoplastic astrocytes were often, but not invariably, stained for NSE. The fine structural localization of NSE in neoplastic cells suggests that the conversion of 2-D-glycerophosphate to phosphoenolpyruvate by enolase may occur on the membrane and, in the case of astrocytic tumours, on the cytoplasmic filaments as well as in the cytoplasm. When cells which contain only the non-neuronal form of enolase (NNE) transform to neoplastic cells, they may acquire the ability to produce NSE. This presumably enables them to accommodate the increased metabolic demands of neoplasia by allowing them to elude the regulatory controls that are specific for NNE.
对一系列原发性和转移性人脑肿瘤进行免疫细胞化学评估，以确定神经元特异性烯醇化酶（NSE）的电镜定位。所有肿瘤均含有细胞，无论细胞类型如何，这些细胞的细胞质和细胞膜上NSE均呈不规则分布。这与正常中枢神经系统（CNS）细胞的染色模式形成对比，正如先前报道的那样（维诺雷斯等人，1984b），正常CNS细胞仅显示弥漫性细胞质染色，通常与细胞膜无关。在肿瘤中，细胞核内部以及线粒体的嵴和基质始终呈阴性，与正常CNS细胞一样。除了一例低级别纤维性星形细胞瘤外，肿瘤性星形胶质细胞中的细胞质细丝常被NSE染色，但并非总是如此。NSE在肿瘤细胞中的精细结构定位表明，烯醇化酶将2-磷酸甘油转化为磷酸烯醇丙酮酸的过程可能发生在细胞膜上，对于星形细胞瘤而言，也可能发生在细胞质细丝以及细胞质中。当仅含有非神经元形式烯醇化酶（NNE）的细胞转化为肿瘤细胞时，它们可能获得产生NSE的能力。据推测，这使它们能够通过逃避NNE特有的调节控制来适应肿瘤增加的代谢需求。
