Vinores S A, Herman M M, Rubinstein L J
Department of Pathology, University of Virginia School of Medicine, Charlottesville 22908.
Histochem J. 1987 Aug;19(8):438-48. doi: 10.1007/BF01675755.
Neuron-specific (gamma gamma) enolase, a glycolytic enzyme used as a relatively specific marker for normal neurons and neuroendocrine cells, has recently been found in a variety of neoplastic cells and in reactive astrocytes. Its localization was investigated by immunohisto- and electron-immunocyto-chemistry, in the proliferating supportive Schwann cells of a peripheral ganglioneuroblastoma and in the neoplastic Schwann cells of four acoustic tumours. By light microscopy, the neoplastic Schwann cells showed moderate uneven diffuse immunopositivity for enolase. By electron-immunocytochemistry, both types of Schwann cells demonstrated immunopositivity discretely limited to their cell surface membranes. The neoplastic ganglion cells and axons of the ganglioneuroblastoma and the normal neurons and axons included in the schwannomas were, as expected, intensely immunopositive. The visualization of gamma gamma enolase on the cell surface membranes of both neoplastic and non-neoplastic proliferating Schwann cells suggests that increased glycolytic activity may occur on the surface of these proliferating cells irrespective of the nature of the proliferation.
神经元特异性（γγ）烯醇化酶是一种糖酵解酶，用作正常神经元和神经内分泌细胞的相对特异性标志物，最近在多种肿瘤细胞和反应性星形胶质细胞中被发现。通过免疫组织化学和电子免疫细胞化学方法，研究了其在外周神经节神经母细胞瘤增殖性支持性施万细胞以及4例听神经瘤的肿瘤性施万细胞中的定位。光镜下，肿瘤性施万细胞对烯醇化酶呈中度不均匀弥漫性免疫阳性。通过电子免疫细胞化学，两种类型的施万细胞均显示免疫阳性离散地局限于其细胞膜表面。正如预期的那样，神经节神经母细胞瘤的肿瘤性神经节细胞和轴突以及神经鞘瘤中的正常神经元和轴突均呈强免疫阳性。肿瘤性和非肿瘤性增殖性施万细胞膜表面γγ烯醇化酶的可视化表明，无论增殖的性质如何，这些增殖细胞表面可能会出现糖酵解活性增加。
