State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
Sci Total Environ. 2022 Aug 15;834:154947. doi: 10.1016/j.scitotenv.2022.154947. Epub 2022 Mar 30.
The enormous waste of polyethylene terephthalate (PET) plastic has a great negative impact on the ecological environment because of its chemical inertia. To reduce the environmental threat posed by PET plastic, researchers gradually concentrate on the biodegradation of PET plastic. In this study, DuraPETase (DuraPETase-4M) was designed through protein engineering, which can be used to improve the efficiency of PET plastic biodegradation. Based on the DuraPETase, a pair of disulfide bonds (N233C/S282C) was added to improve the thermal stability. Meanwhile, the key region flexibility adjustment (H214S) was proposed to enhance the biodegradation capacity of PET plastic. Additionally, protein surface electrostatic charge optimization (S245R) was adopted to improve the binding ability between enzyme and PET plastic. Based on molecular dynamic simulations (MDs), the rationality of the design was further verified. This study provides a strategy for obtaining high-efficiency PET degradation mutants and a new possibility of environmentally friendly plastic degradation.
聚对苯二甲酸乙二醇酯（PET）塑料因其化学惰性而大量浪费，对生态环境造成了极大的负面影响。为了降低 PET 塑料带来的环境威胁，研究人员逐渐专注于 PET 塑料的生物降解。在这项研究中，通过蛋白质工程设计了一种名为 DuraPETase（DuraPETase-4M）的酶，可用于提高 PET 塑料生物降解的效率。基于 DuraPETase，引入了一对二硫键（N233C/S282C）以提高其热稳定性。同时，提出了关键区域灵活性调整（H214S）以增强 PET 塑料的生物降解能力。此外，采用蛋白质表面静电电荷优化（S245R）以提高酶与 PET 塑料之间的结合能力。通过分子动力学模拟（MDs）进一步验证了设计的合理性。这项研究为获得高效的 PET 降解突变体提供了一种策略，为环保型塑料降解提供了新的可能性。
