Bekmurzayeva Aliya, Ashikbayeva Zhannat, Assylbekova Nazerke, Myrkhiyeva Zhuldyz, Dauletova Ayazhan, Ayupova Takhmina, Shaimerdenova Madina, Tosi Daniele
School of Engineering and Digital Sciences, Nazarbayev University, Nur-Sultan, Kazakhstan; National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan.
School of Engineering and Digital Sciences, Nazarbayev University, Nur-Sultan, Kazakhstan.
Biosens Bioelectron. 2022 Jul 15;208:114217. doi: 10.1016/j.bios.2022.114217. Epub 2022 Mar 26.
Measuring cancer biomarkers at ultralow detection limit and high sensitivity could be a promising tool for early diagnosis, monitoring treatment and post-treatment recurrence. Soluble CD44 is a promising diagnostic and prognostic biomarker in several types of cancer including gastric, colon and breast cancer. Several highly sensitive biosensors have been built to measure this important biomarker. However, they did not reach attomolar level of detection. The aim of this work was to build a biosensor capable of detecting CD44 concentrations down to attomolar (aM) level while measuring it in a wide concentration range. Herein, we demonstrate a biosensor that offers 4 key advantages over existing platforms for CD44 detection: 1) detection of CD44 was carried out in a diluted serum down to attomolar level (4.68 aM) which is about 6 orders of magnitude lower than that of a traditional ELISA; 2) fabrication of the sensor is done in a fast way using inexpensive materials making it a disposable fiber optic biosensor; 3) detection of CD44 was performed in a wide dynamic range previously not shown in other similar biosensors; 4) a proof-of-concept experiment was performed using the biosensor to embed it in a catheter to measure the protein in flow conditions.
在超低检测限和高灵敏度下测量癌症生物标志物可能是早期诊断、监测治疗及治疗后复发的一种有前景的工具。可溶性CD44是包括胃癌、结肠癌和乳腺癌在内的几种癌症中一种有前景的诊断和预后生物标志物。已经构建了几种高灵敏度生物传感器来测量这种重要的生物标志物。然而，它们并未达到阿托摩尔级别的检测限。这项工作的目的是构建一种生物传感器，能够在宽浓度范围内检测低至阿托摩尔（aM）水平的CD44浓度。在此，我们展示了一种生物传感器，它在检测CD44方面比现有平台具有4个关键优势：1）在稀释血清中对CD44进行检测，低至阿托摩尔水平（4.68 aM），这比传统酶联免疫吸附测定法（ELISA）低约6个数量级；2）使用廉价材料以快速方式制造传感器，使其成为一次性光纤生物传感器；3）在宽动态范围内对CD44进行检测，这是其他类似生物传感器以前未实现的；4）使用该生物传感器进行了概念验证实验，将其嵌入导管中以在流动条件下测量蛋白质。
