长链非编码RNA MALAT1/miR-1-3p/CXCR4轴在糖尿病性神经病变患者中失调。

The axis of long non-coding RNA MALAT1/miR-1-3p/CXCR4 is dysregulated in patients with diabetic neuropathy.

作者信息

Ashjari Donya, Karamali Negin, Rajabinejad Misagh, Hassani Seyedeh Sara, Afshar Hezarkhani Leila, Afshari Daryoush, Gorgin Karaji Ali, Salari Farhad, Rezaiemanesh Alireza

机构信息

Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

出版信息

Heliyon. 2022 Mar 24;8(3):e09178. doi: 10.1016/j.heliyon.2022.e09178. eCollection 2022 Mar.

DOI:10.1016/j.heliyon.2022.e09178

PMID:35368523

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8969120/

Abstract

BACKGROUND

Diabetic neuropathy (DN) is a prevalent complication of diabetes mellitus characterized by pain and inflammation. Long non-coding RNAs (lncRNAs) have been associated with DN. This study aimed to investigate transcript levels of Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), microRNA (miR)-1-3p, and C-X-C motif chemokine receptor 4 (CXCR4) in the DN patients and type 2 diabetes mellitus (T2DM) cases without neuropathy.

METHODS

Here, 20 cases with DN and 20 T2DM subjects without neuropathy (as the control group) were included. Total RNA was extracted from peripheral blood mononuclear cells (PBMCs) of all participants. The expression levels of targets were evaluated by Real-time-PCR.

RESULTS

Results showed that MALAT1 (Fold change = 2.47, = 0.03) and CXCR4 (Fold change = 1.65, = 0.023) were significantly upregulated, while miR-1-3p was downregulated (Fold change = 0.9, = 0.028) in whole blood samples from DN patients compared to the control group. A significant correlation was found between transcript levels of MALAT1 and CXCR4 ( = 0.84; < 0.0001).

CONCLUSIONS

This study suggests a possible involvement of the MALAT1/miR-1-3p/CXCR4 axis in the pathogenesis of DN.

摘要

背景

糖尿病性神经病变（DN）是糖尿病常见的并发症，其特征为疼痛和炎症。长链非编码RNA（lncRNA）与DN相关。本研究旨在调查转移相关肺腺癌转录本1（MALAT1）、微小RNA（miR）-1-3p和C-X-C基序趋化因子受体4（CXCR4）在DN患者及无神经病变的2型糖尿病（T2DM）患者中的转录水平。

方法

本研究纳入20例DN患者和20例无神经病变的T2DM患者（作为对照组）。从所有参与者的外周血单个核细胞（PBMC）中提取总RNA。通过实时定量PCR评估靶标的表达水平。

结果

结果显示，与对照组相比，DN患者全血样本中MALAT1（倍数变化=2.47，P=0.03）和CXCR4（倍数变化=1.65，P=0.023）显著上调，而miR-1-3p下调（倍数变化=0.9，P=0.028）。发现MALAT1和CXCR4的转录水平之间存在显著相关性（r=0.84；P<0.0001）。