Xu Jingnan, Ma Xueyan, Bai Chen, Jiang Xin, Huang Ling, Gao Fei, Li Yini, Yu He, Liu Tiegang, Gu Xiaohong
Beijing University of Chinese Medicine, Beijing 100029, China.
Evid Based Complement Alternat Med. 2022 Mar 23;2022:3034714. doi: 10.1155/2022/3034714. eCollection 2022.
We intended to explore the mechanism of decoction in the treatment of lipopolysaccharide (LPS)-induced pneumonia from the perspective of intestinal flora.
Thirty Sprague-Dawley rats were randomly assigned to the blank control group (N), the pneumonia group (P), and the decoction group (PT). The rat pneumonia model was established using LPS inhalation (0.5 mg/mL, 5 mL, 30 min/day, 3 days). decoction was administered intragastrically (2 mL/100 g, 3 days). Lung tissue pathology, organ indexes, serum inflammatory factors, tumor necrosis factor-alpha (TNF-), and intestinal flora changes were measured.
Lung tissue inflammation was prevented by decoction. IL-6 levels showed a higher tendency to be higher, and IL-12 and TNF- were significantly higher in the PT group than in the P group. The structure of the intestinal flora in the P differed from that in the N. The relative abundance of 10 out of 12 microflora was significantly higher in the P group than in the N and PT groups. In the PT group, the structure and the distribution of microbial groups were like those of the N group.
decoction inhibited LPS-induced lung and systemic inflammation in rats and may help the intestinal flora restore equilibrium by inhibiting the colonization of pathogenic bacteria and adjusting the ratio between probiotics and pathogenic bacteria. Intestinal flora may serve as a mediator of decoction's effect on LPS-induced pneumonia.
我们旨在从肠道菌群的角度探讨[方剂名称]汤剂治疗脂多糖（LPS）诱导的肺炎的机制。
将30只Sprague-Dawley大鼠随机分为空白对照组（N）、肺炎组（P）和[方剂名称]汤剂组（PT）。采用吸入LPS（0.5mg/mL，5mL，每天30分钟，共3天）建立大鼠肺炎模型。[方剂名称]汤剂灌胃给药（2mL/100g，共3天）。检测肺组织病理学、器官指数、血清炎症因子、肿瘤坏死因子-α（TNF-α）及肠道菌群变化。
[方剂名称]汤剂可预防肺组织炎症。PT组IL-6水平呈较高升高趋势，IL-12和TNF-α水平显著高于P组。P组肠道菌群结构与N组不同。P组12种微生物中的10种相对丰度显著高于N组和PT组。PT组微生物群的结构和分布与N组相似。
[方剂名称]汤剂可抑制LPS诱导的大鼠肺部和全身炎症，可能通过抑制病原菌定植、调节益生菌与病原菌比例，帮助肠道菌群恢复平衡。肠道菌群可能是[方剂名称]汤剂对LPS诱导的肺炎发挥作用的介导因素。
