Department of Surgery, Charles R. Drew University of Medicine and Science, Los Angeles, CA 90059, USA.
Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria 21511, Egypt.
Nutrients. 2021 Nov 4;13(11):3936. doi: 10.3390/nu13113936.
The potential of KDP, a lactic acid bacterial strain of , to enhance the production of mucosal specific immunoglobulin A (IgA) in mice and thereby enhance gut mucosal immunity was examined. KDP is composed of dead cells isolated from the Korean traditional food kimchi. Female BALB/c mice orally received 0.25 mg KDP once daily for 5 weeks and were co-administrated ovalbumin (OVA) for negative control and cholera toxin for positive control. Mice administered KDP exhibited increased secretory IgA (sIgA) contents in the small intestine, Peyer's patches, serum, colon, and lungs as examined by ELISA. KDP also significantly increased the gene expression of , , , , and . In addition, KDP acted as a potent antioxidant, as indicated by its significant inhibitory effects in the range of 16.5-59.4% for DPPH, nitric oxide, maximum total antioxidant capacity, and maximum reducing power. Finally, KDP exhibited potent antimicrobial activity as evidenced by a significant decrease in the growth of 7 samples of gram-negative and gram-positive bacteria and . KDP's adjuvant effect is shown to be comparable to that of cholera toxin. We conclude that KDP can significantly enhance the intestine's secretory immunity to OVA, as well as act as a potent antioxidant and antimicrobial agent. These results suggest that orally administered KDP should be studied in clinical trials for antigen-specific IgA production.
我们研究了 乳酸菌 KDP 增强黏膜特异性免疫球蛋白 A（IgA）产生从而增强肠道黏膜免疫的潜力。KDP 由韩国传统食品泡菜中分离出的死细胞组成。雌性 BALB/c 小鼠每天口服 0.25mg KDP，连续 5 周，并同时给予卵清蛋白（OVA）作为阴性对照和霍乱毒素作为阳性对照。通过 ELISA 检测，我们发现给予 KDP 的小鼠小肠、派尔集合淋巴结、血清、结肠和肺部的分泌型 IgA（sIgA）含量增加。KDP 还显著增加了 、 、 、 和 的基因表达。此外，KDP 具有很强的抗氧化作用，其对 DPPH、一氧化氮、最大总抗氧化能力和最大还原能力的抑制作用范围在 16.5-59.4%之间。最后，KDP 表现出很强的抗菌活性，可显著降低 7 种革兰氏阴性和革兰氏阳性细菌和 的生长。KDP 的佐剂作用与霍乱毒素相当。我们的结论是，KDP 可以显著增强肠道对 OVA 的分泌性免疫，同时具有很强的抗氧化和抗菌作用。这些结果表明，口服 KDP 应在临床试验中进一步研究其产生抗原特异性 IgA 的能力。
