使用库尔特计数器测定细菌细胞体积。
Determination of bacterial cell volume with the Coulter Counter.
作者信息
Kubitschek H E, Friske J A
出版信息
J Bacteriol. 1986 Dec;168(3):1466-7. doi: 10.1128/jb.168.3.1466-1467.1986.
Abstract
Two methods were used to determine mean volumes of cells of Escherichia coli B/rA in both stationary- and exponential-phase cultures, i.e., electronic measurement with a Coulter Counter-Analyzer system and biophysical measurement of the total volume and number of cells in sedimented cell pellets. Within experimental errors, the methods gave the same mean cell volumes.
摘要
采用两种方法测定了处于稳定期和对数期培养的大肠杆菌B/rA细胞的平均体积，即使用库尔特计数器-分析仪系统进行电子测量，以及对沉淀细胞团中的细胞总体积和数量进行生物物理测量。在实验误差范围内，这两种方法得出的平均细胞体积相同。
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