Clinical Medical College and The First Affiliated Hospital of Chengdu Medical College, Si Chuan, China.
No.4 West China Teaching Hospital of Si Chuan University, Si Chuan, China.
J Healthc Eng. 2022 Mar 24;2022:1620802. doi: 10.1155/2022/1620802. eCollection 2022.
Pancreatic cancer (PC) stands out as one of the most lethal cancers. Due to late diagnosis, only a fraction of patients can be resected. Although it still has significant adverse effects and poor results, the treatment is connected with better overall survival than the prior treatment. Thus, new alternative therapy for advanced PC is needed. . The impact of 10058-F4 and curcumin combination therapy on apoptosis and cell growth in SW1990 pancreatic cancer cells were determined in vitro using the CCK-8 assay and flow cytometry of Annexin V-FITC/PI, and the in vivo antitumor effect was determined utilizing SW1990-bearing pancreatic tumor mouse models induced by subcutaneous implantation.
At concentrations of (10 mol/L+2 mol/L), 10058-F4+curcumin obtained the highest rate of SW1990 cell death, and they had a beneficial effect on SW1990 pancreatic tumor-bearing animals. Furthermore, c-Myc, Akt phosphorylation, and the expression of apoptosis-related molecular were reduced, and the combination therapy modified the expression of apoptosis-related molecular.
In vitro and in vivo, the combination of 10058-F4 plus curcumin has antipancreatic cancer actions that are substantially effective.
胰腺癌（PC）是最致命的癌症之一。由于诊断较晚，只有一小部分患者可以进行手术切除。尽管这种治疗方法仍有显著的不良反应和较差的结果，但与之前的治疗相比，它与更好的总生存期相关。因此，需要新的治疗晚期 PC 的替代疗法。本文旨在研究 10058-F4 和姜黄素联合治疗对体外培养的 SW1990 胰腺癌细胞凋亡和细胞生长的影响，采用 CCK-8 法和 Annexin V-FITC/PI 流式细胞术检测 10058-F4 和姜黄素联合治疗对 SW1990 胰腺癌细胞凋亡和细胞生长的影响，利用皮下接种 SW1990 胰腺癌细胞建立胰腺癌荷瘤小鼠模型，检测体内抗肿瘤作用。结果：在浓度为（10 μmol/L+2 μmol/L）时，10058-F4+姜黄素获得了最高的 SW1990 细胞死亡率，对 SW1990 胰腺肿瘤荷瘤动物有有益的作用。此外，c-Myc、Akt 磷酸化和凋亡相关分子的表达减少，联合治疗改变了凋亡相关分子的表达。结论：在体外和体内，10058-F4 联合姜黄素具有明显有效的抗胰腺癌作用。
