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超亲水 PLGA 接枝 PVP/PC 纳米纤维膜预防硬膜外粘连。

Superhydrophilic PLGA-Graft-PVP/PC Nanofiber Membranes for the Prevention of Epidural Adhesion.

机构信息

Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu, People's Republic of China.

Department of Orthopedics, The Second Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China.

出版信息

Int J Nanomedicine. 2022 Mar 25;17:1423-1435. doi: 10.2147/IJN.S356250. eCollection 2022.

DOI:10.2147/IJN.S356250
PMID:35369033
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8964670/
Abstract

BACKGROUND

The frequent occurrence of failed back surgery syndrome (FBSS) seriously affects the quality of life of postoperative lumbar patients. Epidural adhesion is the major factor in FBSS.

PURPOSE

A safe and effective antiadhesion material is urgently needed.

METHODS

A superhydrophilic PLGA-g-PVP/PC nanofiber membrane (NFm) was prepared by electrospinning. FTIR was performed to identify its successful synthesis. Scanning electron microscopy, thermogravimetric analysis, differential scanning calorimetry, and water contact angle measurement were performed. CCK-8 assays were performed in primary rabbit fibroblasts (PRFs) and RAW264.7 cells to explore the cytotoxicity of PLGA-g-PVP/PC NFm. Calcein-AM/PI staining was used to measure the adhesion status in PRFs. ELISA was performed to measure the concentrations of TNF-α and IL-10 in RAW264.7 cells. In addition, the anti-epidural adhesion efficacy of the PLGA-g-PVP/PC NFm was determined in a rabbit model of lumbar laminectomy.

RESULTS

The PLGA-g-PVP/PC NFm exhibited ultrastrong hydrophilicity and an appropriate degradation rate. Based on the results of the CCK-8 assays, PLGA-g-PVP/PC NFm had no cytotoxicity to PRFs and RAW264.7 cells. Calcein-AM/PI staining showed that PLGA-g-PVP/PC NFm could inhibit PRF adhesion. ELISAs showed that PLGA-g-PVP/PC NFm could attenuate lipopolysaccharide-induced macrophage activation. In vivo experiments further confirmed the favorable anti-epidural adhesion effect of PLGA-g-PVP/PC NFm and the lack of a strong inflammatory response.

CONCLUSION

In this study, PLGA-g-PVP/PC NFm was developed successfully to provide a safe and effective physical barrier for preventing epidural adhesion. PLGA-g-PVP/PC NFm provides a promising strategy for preventing postoperative adhesion and has potential for clinical translation.

摘要

背景

频繁发生的失败性腰椎手术后综合征（FBSS）严重影响了术后腰椎患者的生活质量。硬膜外粘连是 FBSS 的主要因素。

目的

迫切需要一种安全有效的防粘连材料。

方法

通过静电纺丝制备了超亲水 PLGA-g-PVP/PC 纳米纤维膜（NFm）。通过傅里叶变换红外光谱（FTIR）对其成功合成进行了鉴定。进行了扫描电子显微镜（SEM）、热重分析（TGA）、差示扫描量热法（DSC）和水接触角测量。在原代兔成纤维细胞（PRFs）和 RAW264.7 细胞中进行 CCK-8 测定，以研究 PLGA-g-PVP/PC NFm 的细胞毒性。使用钙黄绿素 AM/PI 染色法测量 PRFs 的黏附状态。通过酶联免疫吸附试验（ELISA）测定 RAW264.7 细胞中 TNF-α和 IL-10 的浓度。此外，还在兔腰椎板切除术模型中确定了 PLGA-g-PVP/PC NFm 的抗硬膜外粘连效果。

结果

PLGA-g-PVP/PC NFm 表现出超强的亲水性和适当的降解率。根据 CCK-8 测定的结果，PLGA-g-PVP/PC NFm 对 PRFs 和 RAW264.7 细胞没有细胞毒性。钙黄绿素 AM/PI 染色显示 PLGA-g-PVP/PC NFm 可以抑制 PRF 黏附。ELISA 结果表明，PLGA-g-PVP/PC NFm 可以减轻脂多糖诱导的巨噬细胞活化。体内实验进一步证实了 PLGA-g-PVP/PC NFm 良好的抗硬膜外粘连效果和缺乏强烈的炎症反应。

结论

在这项研究中，成功开发了 PLGA-g-PVP/PC NFm，为防止硬膜外粘连提供了一种安全有效的物理屏障。PLGA-g-PVP/PC NFm 为预防术后粘连提供了一种有前景的策略，具有临床转化的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/4239b115c21a/IJN-17-1423-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/695bd4668a72/IJN-17-1423-g0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/c37d0cd27d30/IJN-17-1423-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/bfe5de90e0d7/IJN-17-1423-g0006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/4239b115c21a/IJN-17-1423-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/695bd4668a72/IJN-17-1423-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/896dae77cb7b/IJN-17-1423-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/edfd73e10499/IJN-17-1423-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/d5f11d081af1/IJN-17-1423-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/c37d0cd27d30/IJN-17-1423-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/bfe5de90e0d7/IJN-17-1423-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/4fa66f290b27/IJN-17-1423-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d3e/8964670/99a8f0766eab/IJN-17-1423-g0008.jpg
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