Chen Shih-Hsien, Chen Chih-Hao, Shalumon K T, Chen Jyh-Ping
Department of Chemical and Materials Engineering, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taiwan, Republic of China.
Department of Chemical and Materials Engineering, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taiwan, Republic of China ; Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taiwan, Republic of China.
Int J Nanomedicine. 2014 Aug 22;9:4079-92. doi: 10.2147/IJN.S67931. eCollection 2014.
Peritendinous adhesion is one of the common complications encountered after tendon injury and subsequent surgery, and it can be minimized by introducing a physical barrier between the injured site and the surrounding tissue. An electrospun hyaluronic acid-grafted poly(caprolactone) (PCL-g-HA) nanofibrous membrane (NFM) is proposed as an alternative to current antiadhesion barrier films. HA is covalently grafted to surface-aminolyzed PCL nanofibers, using carbodiimide as the coupling agent. Pristine PCL and PCL-g-HA NFMs were characterized by scanning electron microscopy, thermogravimetric analysis, X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy, and mechanical testing. In vitro cell culture with fibroblasts showed that PCL-g-HA NFMs reduced cellular adhesion on the membrane surface while maintaining cell proliferation. Animal experiments using a rabbit flexor digitorum profundus tendon model confirmed the efficacy of PCL-g-HA in reducing peritendinous adhesion, based on gross observation, histology, joint flexion-angle measurements, gliding tests, and biomechanical evaluation.
肌腱周围粘连是肌腱损伤及后续手术后常见的并发症之一，通过在损伤部位与周围组织之间引入物理屏障可将其降至最低。有人提出将静电纺丝的透明质酸接枝聚己内酯（PCL-g-HA）纳米纤维膜（NFM）作为当前抗粘连屏障膜的替代品。使用碳二亚胺作为偶联剂，将HA共价接枝到表面氨基化的PCL纳米纤维上。通过扫描电子显微镜、热重分析、X射线光电子能谱、傅里叶变换红外光谱和力学测试对原始PCL和PCL-g-HA NFM进行了表征。用成纤维细胞进行的体外细胞培养表明，PCL-g-HA NFM在保持细胞增殖的同时减少了细胞在膜表面的粘附。使用兔指深屈肌腱模型进行的动物实验，基于大体观察、组织学、关节屈曲角度测量、滑动试验和生物力学评估，证实了PCL-g-HA在减少肌腱周围粘连方面的有效性。
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