培养菌丝体的标准化提取物可抑制感染机制。
A Standardized Extract of Cultured Mycelium Inhibits Infectivity Mechanisms.
作者信息
Tena-Garitaonaindia Mireia, Ceacero-Heras Diego, Montoro María Del Mar Maldonado, de Medina Fermín Sánchez, Martínez-Augustin Olga, Daddaoua Abdelali
机构信息
Department of Biochemistry and Molecular Biology II, Pharmacy School, University of Granada, Granada, Spain.
Clinical Analysis Service, Hospital Campus de la Salud, Granada, Spain.
出版信息
Front Microbiol. 2022 Mar 16;13:814448. doi: 10.3389/fmicb.2022.814448. eCollection 2022.
The priority pathogen list of the World Health Organization classified as the second top critical pathogen. Hence, the development of novel antibacterial strategies to tackle this bacterium is highly necessary. Herein we explore the potential antibacterial effect of a standardized extract of cultured mycelium of (AHCC) on . AHCC was found to inhibit the growth rate and biofilm formation of strain PAO1. No change in swarming was observed, but AHCC hampered swimming and twitching motility. In accordance, a decreased expression of metabolism, growth, and biofilm formation genes was shown. AHCC also diminished the levels of exotoxin A and bacteria inside IEC18 cells and the secretion of IL-6, IL-10 and TNF by infected macrophages. This effect was related to a reduced phosphorylation of MAPKs and to bacteria internalization. Taken together, our data suggest that AHCC has a potential role to prevent infections and may lead to the development of new therapies.
世界卫生组织的重点病原体清单将其列为第二大关键病原体。因此，开发新型抗菌策略来对付这种细菌非常必要。在此，我们探讨了培养的（活性己糖相关化合物）菌丝体标准化提取物对的潜在抗菌作用。发现活性己糖相关化合物可抑制PAO1菌株的生长速率和生物膜形成。未观察到群体运动的变化，但活性己糖相关化合物阻碍了游动和颤动运动。相应地，显示出代谢、生长和生物膜形成基因的表达降低。活性己糖相关化合物还降低了IEC18细胞内的外毒素A和细菌水平以及感染巨噬细胞分泌的IL-6、IL-10和TNF。这种作用与丝裂原活化蛋白激酶的磷酸化减少和细菌内化有关。综上所述，我们的数据表明活性己糖相关化合物在预防感染方面具有潜在作用，并可能导致新疗法的开发。
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