Al-Jameel Waseem, Al-Mahmood S S, Al-Saidya A M
Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
Vet World. 2022 Jan;15(1):10-15. doi: 10.14202/vetworld.2022.10-15. Epub 2022 Jan 7.
Squamous cell carcinoma (SCC) is the most common form of carcinoma in cattle. Histopathological grading systems have been utilized over several decades for estimating the malignancy of cattle SCCs. This study aimed to detect p53 and Mdm2 expression in different SCC cases in cattle and correlate their expression with the SCC histopathological grading.
Cattle SCC cases were collected at the Veterinary Teaching Hospital in Nineveh. The SCC grading system categorized the cases histologically based on their differentiation grade into three groups: Well, moderately, and poorly differentiated. The SCC cases were subsequently verified for p53 and Mdm2 immunoexpression.
Fourteen of 16 examined cattle SCC samples tested positive for p53 expression. Moreover, 15 out of the 16 SCC samples tested positive for Mdm2 expression. The increased immunoreactivity of both p53 and Mdm2 was associated with a poor histological grading of the cattle SCC. There is a positive correlation between the nuclear expression of p53 and Mdm2, and the degree of differentiation and the number of mitotic figures in the examined cattle SCC samples.
Our results demonstrate an increased p53 and Mdm2 expression in cattle SCC cases characterized by poor histopathological grading, thus suggesting an essential role of these molecules in the development of moderately and poorly differentiated SCC in cattle.
鳞状细胞癌（SCC）是牛最常见的癌形式。几十年来，组织病理学分级系统一直被用于评估牛SCC的恶性程度。本研究旨在检测牛不同SCC病例中p53和Mdm2的表达，并将它们的表达与SCC组织病理学分级相关联。
在尼尼微的兽医教学医院收集牛SCC病例。SCC分级系统根据病例的分化程度将其组织学分类为三组：高分化、中分化和低分化。随后对SCC病例进行p53和Mdm2免疫表达验证。
16份检测的牛SCC样本中有14份p53表达呈阳性。此外，16份SCC样本中有15份Mdm2表达呈阳性。p53和Mdm2免疫反应性的增加与牛SCC的组织学分级差有关。在所检测的牛SCC样本中，p53和Mdm2的核表达与分化程度和有丝分裂象数量之间存在正相关。
我们的结果表明，在组织病理学分级差的牛SCC病例中，p53和Mdm2表达增加，因此提示这些分子在牛中分化和低分化SCC的发生发展中起重要作用。
