Sözmen M, Devrim A K, Sudağıdan M, Kabak Y B, Beytut E, Özba B
Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey.
Department of Biochemistry, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey.
J Comp Pathol. 2019 Jul;170:60-69. doi: 10.1016/j.jcpa.2019.05.008. Epub 2019 Jun 22.
Bovine ocular squamous cell carcinoma (BOSCC) is the most common and economically significant neoplasm of the eye in cattle. This study investigated the role of angiogenic growth factors in the pathogenesis of BOSCC. Eighteen cases of BOSCC were classified histopathologically according to the degree of differentiation. Normal upper and lower eyelids and third eyelids collected from the right and left eyes of six healthy cattle aged 1-3 years, that had been presented for slaughter to abattoirs, served as controls. Transcription of genes encoding the angiogenic growth factors vascular endothelial growth factor-C (VEGF-C), basic fibroblast growth factor (bFGF), platelet-derived growth factor-C (PDGF-C) and platelet-derived growth factor receptor-α (PDGFR-α) was determined by quantitative real-time polymerase chain reaction (RT-PCR) in tissue obtained from paraffin wax blocks. Immunohistochemistry (IHC) was utilized to detect intensity of expression and tissue distribution of these growth factors. IHC results revealed that bFGF and PDGF-C were elevated significantly (P >0.05) and VEGF-C expression was decreased in BOSCC compared with healthy control tissue. PDGR-α expression was elevated; however, the difference, compared with control tissues, was not significant. RT-PCR results showed an inverse relationship to the results of IHC; where protein levels were elevated their corresponding mRNA levels were decreased or vice-versa. Angiogenic regulators therefore appear to play a role in the pathogenesis of BOSCC.
牛眼鳞状细胞癌（BOSCC）是牛眼部最常见且在经济上具有重要意义的肿瘤。本研究调查了血管生成生长因子在BOSCC发病机制中的作用。根据分化程度对18例BOSCC进行了组织病理学分类。从6头1 - 3岁健康牛的左右眼收集的正常上、下眼睑及第三眼睑，这些牛已送至屠宰场准备屠宰，用作对照。通过定量实时聚合酶链反应（RT-PCR）测定石蜡块组织中编码血管生成生长因子血管内皮生长因子-C（VEGF-C）、碱性成纤维细胞生长因子（bFGF）、血小板衍生生长因子-C（PDGF-C）和血小板衍生生长因子受体-α（PDGFR-α）的基因转录情况。利用免疫组织化学（IHC）检测这些生长因子的表达强度和组织分布。免疫组织化学结果显示，与健康对照组织相比，BOSCC中bFGF和PDGF-C显著升高（P>0.05），VEGF-C表达降低。PDGR-α表达升高；然而，与对照组织相比，差异不显著。RT-PCR结果与免疫组织化学结果呈相反关系；蛋白质水平升高时，其相应的mRNA水平降低，反之亦然。因此，血管生成调节因子似乎在BOSCC的发病机制中发挥作用。
