Robinson J Paul
Purdue University, West Lafayette, IN 47907, USA.
Biotechniques. 2022 Apr;72(4):159-169. doi: 10.2144/btn-2022-0005. Epub 2022 Apr 4.
Flow cytometry is a single-cell technology that measures scatter and fluorescence to establish a set of unique cellular properties. Flow cytometry is used in many areas of science, in particular biotechnology and medicine, but also in industrial applications. Flow cytometry can identify multiple phenotypic subsets from a mixture, select a single cell and even isolate that cell by a process called cell sorting. The field is currently undergoing dramatic changes. We are moving rapidly from the polychromic flow cytometry that has been the go-to technology for 45 years to spectral flow cytometry, which is now the most significant change in nearly half a century of flow cytometry. With change comes opportunity. Even spectral flow cytometry will morph into second-generation spectral flow cytometry within 5 years. New, exciting features will open up molecular diagnostics and physiology to flow cytometry.
流式细胞术是一种单细胞技术，它通过测量散射光和荧光来确定一组独特的细胞特性。流式细胞术在许多科学领域都有应用，特别是生物技术和医学领域，也用于工业应用。流式细胞术可以从混合物中识别多个表型亚群，选择单个细胞，甚至通过一种称为细胞分选的过程分离出该细胞。该领域目前正在经历巨大的变革。我们正迅速从45年来一直作为首选技术的多色流式细胞术转向光谱流式细胞术，这是近半个世纪以来流式细胞术最重大的变革。变革带来机遇。即使是光谱流式细胞术在5年内也将演变成第二代光谱流式细胞术。新的、令人兴奋的特性将为流式细胞术开启分子诊断和生理学的大门。
