Wei He, Lian Yun, Li Jinying, Li Haichao, Song Qijian, Wu Yongkang, Lei Chenfang, Wang Shiwei, Zhang Hui, Wang Jinshe, Lu Weiguo
Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Sciences/National Centre for Plant Breeding/Zhengzhou Subcenter of National Soybean Improvement Center/Key Laboratory of Oil Crops in Huanghuaihai Plains of Ministry of Agriculture, Zhengzhou, China.
Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, United States.
Front Plant Sci. 2022 Mar 15;13:860034. doi: 10.3389/fpls.2022.860034. eCollection 2022.
Soybean cyst nematode (SCN; Ichinohe) is a highly destructive pathogen for soybean production worldwide. The use of resistant varieties is the most effective way of preventing yield loss. Handou 10 is a commercial soybean variety with desirable agronomic traits and SCN resistance, however genes underlying the SCN resistance in the variety are unknown. An F recombinant inbred line (RIL) population derived from a cross between Zheng 9525 (susceptible) and Handou 10 was developed and its resistance to SCN HG type 2.5.7 (race 1) and 1.2.5.7 (race 2) was identified. We identified seven quantitative trait loci (QTLs) with additive effects. Among these, three QTLs on Chromosomes 7, 8, and 18 were resistant to both races. These QTLs could explain 1.91-7.73% of the phenotypic variation of SCN's female index. The QTLs on chromosomes 8 and 18 have already been reported and were most likely overlapped with and loci, respectively. However, the QTL on chromosome 7 was novel. Candidate genes for the three QTLs were predicted through genes functional analysis and transcriptome analysis of infected roots of Handou 10 vs. Zheng 9525. Transcriptome analysis performed also indicated that the plant-pathogen interaction played an important role in the SCN resistance for Handou 10. The information will facilitate SCN-resistant gene cloning, and the novel resistant gene will be a source for improving soybeans' resistance to SCN.
大豆胞囊线虫（SCN；北野）是全球大豆生产中极具破坏性的病原体。使用抗性品种是防止产量损失的最有效方法。邯豆10是一个具有优良农艺性状和抗SCN特性的商业大豆品种，然而该品种抗SCN的潜在基因尚不清楚。构建了一个由郑9525（感病）和邯豆10杂交衍生的F重组自交系（RIL）群体，并鉴定了其对SCN HG 2.5.7型（1号生理小种）和1.2.5.7型（2号生理小种）的抗性。我们鉴定出了7个具有加性效应的数量性状位点（QTL）。其中，位于7号、8号和18号染色体上的3个QTL对两个生理小种均具有抗性。这些QTL可以解释SCN雌虫指数表型变异的1.91%-7.73%。位于8号和18号染色体上的QTL已被报道，分别最有可能与 和 位点重叠。然而，位于7号染色体上的QTL是新发现的。通过对邯豆10和郑9525受侵染根系进行基因功能分析和转录组分析，预测了这3个QTL的候选基因。转录组分析还表明，植物-病原体相互作用在邯豆10对SCN的抗性中起重要作用。这些信息将有助于SCN抗性基因的克隆，而新的抗性基因将成为提高大豆对SCN抗性的来源。
