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用于过氧化物酶组织化学的二氨基联苯胺反应终产物的高级强化。

High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry.

作者信息

Gallyas F, Görcs T, Merchenthaler I

出版信息

J Histochem Cytochem. 1982 Feb;30(2):183-4. doi: 10.1177/30.2.7061820.

DOI:10.1177/30.2.7061820
PMID:7061820
Abstract

A simple and reliable method is described for the intensification of the end-product of the diaminobenzidine reaction demonstrating peroxidase activity. After completing the diaminobenzidine reaction, the preparations to be intensified are immersed first in thioglycolic acid solution, then in distilled water, and finally in a special physical developer containing silver nitrate.

摘要

本文描述了一种简单可靠的方法,用于增强二氨基联苯胺反应终产物以显示过氧化物酶活性。在完成二氨基联苯胺反应后,待增强的制剂先浸入巯基乙酸溶液中,然后浸入蒸馏水中,最后浸入含有硝酸银的特殊物理显影剂中。

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High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry.用于过氧化物酶组织化学的二氨基联苯胺反应终产物的高级强化。
J Histochem Cytochem. 1982 Feb;30(2):183-4. doi: 10.1177/30.2.7061820.
2
Metal-catalyzed oxidation renders silver intensification selective. Applications for the histochemistry of diaminobenzidine and neurofibrillary changes.金属催化氧化使银强化具有选择性。用于二氨基联苯胺组织化学和神经原纤维变化的应用。
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Heavy metal intensification of DAB-based HRP reaction product.基于二氨基联苯胺(DAB)的辣根过氧化物酶(HRP)反应产物的重金属强化
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A simple enhancement method for the silver-gold-intensified diaminobenzidine reaction in the light microscopic immunoperoxidase technique.光镜免疫过氧化物酶技术中银金强化二氨基联苯胺反应的一种简单增强方法。
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Activated charcoal as a adsorbent of oxidized 3,3'-diaminobenzidine in peroxidase cytochemistry.活性炭作为过氧化物酶细胞化学中氧化型3,3'-二氨基联苯胺的吸附剂。
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An assessment of the DAB methods for cytochemical detection of catalase and peroxidase.用于过氧化氢酶和过氧化物酶细胞化学检测的二氨基联苯胺(DAB)方法的评估
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Diaminobenzidine cytochemistry in unfixed human epidermis: a marker for epidermal differentiation and for mitochondria.未固定人表皮中的二氨基联苯胺细胞化学:表皮分化和线粒体的标志物
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[Diaminobenzidine leukocyte cytochemistry: influence of fixation (author's transl)].二氨基联苯胺白细胞细胞化学:固定的影响(作者译)
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The comparative effectiveness of the "brown and blue reactions" for tracing neuronal processes of cells injected intracellularly with horseradish peroxidase.“棕色和蓝色反应”用于追踪用辣根过氧化物酶进行细胞内注射的细胞的神经元突起的比较有效性。
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Histochemistry. 1977 Dec 28;54(4):331-7. doi: 10.1007/BF00508275.

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