Ashman R B
J Immunol Methods. 1986 Nov 20;94(1-2):209-14. doi: 10.1016/0022-1759(86)90235-8.
The candidacidal activity of mouse neutrophils was determined by a colorimetric method, using a tetrazolium dye that detects living, but not dead, yeast cells. The assay is carried out in microtitre trays, read using a multiwell scanning spectrophotometer (ELISA reader), and can be analysed by computer.
采用比色法,利用一种能检测活酵母细胞而非死酵母细胞的四氮唑染料,测定小鼠中性粒细胞的杀念珠菌活性。该检测在微量滴定板中进行,使用多孔扫描分光光度计(酶标仪)读取数据,并且可通过计算机进行分析。