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泰国南部沙敦府拉努区用于诊断蛔虫和钩虫感染的改良非营养琼脂平板培养法

Modified Nonnutrient Agar Plate Culture for the Diagnosis of and Hookworm Infections in La-Ngu District, Satun Province, Southern Thailand.

作者信息

Kitvatanachai Sirima, Kritsiriwutthinan Kanyanan, Taylor Aree, Rhongbutsri Pochong

机构信息

Faculty of Medical Technology, Rangsit University, Pathumthani 12000, Thailand.

Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand.

出版信息

J Parasitol Res. 2022 Mar 25;2022:1117400. doi: 10.1155/2022/1117400. eCollection 2022.

Abstract

Due to the low prevalence and light intensity of (, Ss) and hookworm (HW) in Thailand, an increase in the efficacy of detection with the appropriate diagnosis is needed. This cross-sectional survey is aimed at using modified nonnutrient agar plate culture (mNNAPC) to assess the prevalence of and hookworm infections and to report intestinal parasitic infections in La-Ngu villagers, Satun Province in Southern Thailand. We used wet smear, modified Harada-Mori filter paper culture (mHMFPC), and mNNAPC to investigate 204 villagers (4 villages) between August 2016 and January 2017. The combination of mHMFPC and mNNAPC raised the prevalence of and hookworm infections among the study population from 3.4% and 1.0% to 6.9% and 2.5%, respectively. There were no significant differences between demographic characteristics and these infections ( < 0.05). Three types of protozoal infections, spp. (2.9%), -like (0.5%), and (0.5%), and 3 species of helminthic infections, (6.9%) (14 cases), hookworm 2.5%, and 0.5%, were demonstrated in this area. The mNNAPC showed the highest efficacy in detecting both parasites (Ss 92.9% and HW 80%), whereas the wet smear detected none.

摘要

由于泰国粪类圆线虫(Ss)和钩虫(HW)的感染率较低且感染强度较轻,因此需要通过适当的诊断方法提高检测效率。这项横断面调查旨在使用改良的无营养琼脂平板培养法(mNNAPC)评估泰国南部沙敦府拉恩古村村民的粪类圆线虫和钩虫感染率,并报告肠道寄生虫感染情况。我们在2016年8月至2017年1月期间,使用湿涂片法、改良的原田-森滤纸培养法(mHMFPC)和mNNAPC对204名村民(来自4个村庄)进行了调查。mHMFPC和mNNAPC相结合,使研究人群中粪类圆线虫和钩虫感染率分别从3.4%和1.0%提高到了6.9%和2.5%。人口统计学特征与这些感染之间没有显著差异(P<0.05)。该地区共发现3种原生动物感染,即隐孢子虫属(2.9%)、贾第虫样(0.5%)和微孢子虫(0.5%),以及3种蠕虫感染,即粪类圆线虫(6.9%,14例)、钩虫2.5%和蛔虫0.5%。mNNAPC在检测这两种寄生虫方面显示出最高的效率(粪类圆线虫为92.9%,钩虫为80%),而湿涂片法未检测到任何寄生虫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1238/8975711/a3a44c5d0d3c/JPR2022-1117400.001.jpg

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