Mengist Hylemariam Mihiretie, Demeke Gebreselassie, Zewdie Olifan, Belew Adugna
Department of Medical Laboratory Sciences, College of Health Sciences, Debre Markos University, P.O. Box: 269, Debre Markos, Ethiopia.
Department of Medical Laboratory Sciences, College of Health Sciences, Wollega University, P.O. Box: 395, Nekemte, Ethiopia.
BMC Res Notes. 2018 May 4;11(1):276. doi: 10.1186/s13104-018-3380-z.
The aim of this study was to evaluate the diagnostic performance of direct wet mount microscopy compared to formalin ether concentration (FEC) technique in detecting intestinal helminths in pregnant women.
The total prevalence of intestinal helminths was 18.8% (70/372) by direct wet mount microscopy and 24.7% (92/372) by FEC technique (P < 0.001). The sensitivity, negative predictive value (NPV) and test efficiency (TE) of direct wet mount microscopy in diagnosing intestinal helminths was 76, 92.7 and 94%, respectively. The sensitivity of direct w et mount microscopy was very low in detecting ova of Hymenolepis nana. The two methods showed excellent agreement in detecting ova of Hook worm and Ascaris lumbricoides (Kappa > 0.81) but they fairly agreed in detecting ova of Hymenolepis nana (Kappa = 0.39). Intestinal helminths were underdiagnosed and the total diagnostic performance of direct wet mount microscopy was significantly poor in detecting intestinal helminths as compared to FEC technique. Routine use of FEC method is recommended for the diagnosis of intestinal helminths in pregnant women.
本研究旨在评估直接湿片显微镜检查与福尔马林乙醚浓缩（FEC）技术相比，在检测孕妇肠道蠕虫方面的诊断性能。
直接湿片显微镜检查显示肠道蠕虫的总患病率为18.8%（70/372），FEC技术为24.7%（92/372）（P < 0.001）。直接湿片显微镜检查诊断肠道蠕虫的敏感性、阴性预测值（NPV）和检测效率（TE）分别为76%、92.7%和94%。直接湿片显微镜检查在检测微小膜壳绦虫卵时敏感性非常低。两种方法在检测钩虫卵和蛔虫卵方面显示出极好的一致性（Kappa > 0.81），但在检测微小膜壳绦虫卵方面一致性一般（Kappa = 0.39）。肠道蠕虫存在漏诊情况，与FEC技术相比，直接湿片显微镜检查在检测肠道蠕虫方面的总体诊断性能明显较差。建议常规使用FEC方法诊断孕妇肠道蠕虫。
