Li Jingyun, Chen Juan, Wang Lu, Lin Yan, Zhang Xian, Liu Jian, Wang Fangbin
Department of Food and Biotechnology, School of Food and Biological Engineering, Hefei University of Technology, Baohe District, Hefei, Anhui, PR China.
J Basic Microbiol. 2022 May;62(5):584-592. doi: 10.1002/jobm.202200054. Epub 2022 Apr 3.
l-Fucose, as a monosaccharide in nature, plays a crucial role in bacteria colonization. Escherichia coli (E. coli), as a common microorganism in environment, utilize bacterial flagellar motor to drive the rotation of flagella, which is regulated by chemotactic signal transduction signals. Yet the effect of l-fucose to bacterial motility remains unclear. The effect of l-fucose on the swimming motility of bacteria was investigated from the level of single flagellar motor to individual cell and cell population by employing a bead assay, a high-throughput 2D tracking assay and a high-throughput dark-field flicker microscopy. The results showed that the swimming motility of the bacteria cultured with l-fucose was decreased, while the tumble frequency increased. Furthermore, the behavioral alterations of bacteria affected by l-fucose were directly reveled by measuring the cell distribution of bacteria swimming near surfaces and bacterial surface adhesion, suggesting that l-fucose promotes bacterial surface aggregation and surface adhesion. The effect of l-fucose on bacterial swimming motility characterized in this study are consistent with the key role that l-fucose plays in bacterial colonization.
L-岩藻糖作为自然界中的一种单糖，在细菌定殖中起着至关重要的作用。大肠杆菌作为环境中常见的微生物，利用细菌鞭毛马达驱动鞭毛旋转，这一过程受趋化信号转导信号调控。然而，L-岩藻糖对细菌运动性的影响仍不清楚。通过采用磁珠试验、高通量二维跟踪试验和高通量暗场闪烁显微镜，从单个鞭毛马达水平到单个细胞及细胞群体水平，研究了L-岩藻糖对细菌游动性的影响。结果表明，用L-岩藻糖培养的细菌游动性降低，而翻滚频率增加。此外，通过测量靠近表面游动的细菌的细胞分布和细菌表面黏附，直接揭示了受L-岩藻糖影响的细菌行为改变，表明L-岩藻糖促进细菌表面聚集和表面黏附。本研究中所表征的L-岩藻糖对细菌游动性的影响与L-岩藻糖在细菌定殖中所起的关键作用一致。
