School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, China.
J Basic Microbiol. 2023 Aug;63(8):897-908. doi: 10.1002/jobm.202200716. Epub 2023 Apr 14.
The antiglycemic drug metformin, which is widely prescribed as a first-line drug for the treatment of type 2 diabetes, has become a concern for emerging pollutants in natural ecosystems. However, its effects on bacterial swimming motility remain unclear. In this study, we showed that metformin promotes bacterial surface aggregation by tracking swimming and by measuring the density distribution of Escherichia coli cultured with metformin near a surface in a homogeneous environment. Flagella are essential for the promotion of bacterial surface aggregation by metformin. Swimming motility, which is mediated by flagella, determines bacterial surface aggregation. The promotion of bacterial surface aggregation by metformin is caused by a reduction in swimming motility, which is governed by a decrease in the proton motive force. Our results reveal that metformin has a pronounced effect on flagellated bacterial motility associated with surface sensing and aggregation.
作为治疗 2 型糖尿病的一线药物，广泛应用的抗糖尿病药物二甲双胍已成为自然生态系统中新兴污染物的关注点。然而，其对细菌游动能力的影响尚不清楚。在这项研究中，我们通过跟踪游动并测量在同质环境中在表面附近培养的大肠杆菌的密度分布，表明二甲双胍通过促进细菌表面聚集来促进细菌游动。二甲双胍促进细菌表面聚集需要依赖鞭毛。由鞭毛介导的游动能力决定了细菌表面聚集。二甲双胍促进细菌表面聚集是由于游动能力降低所致，而这是由质子动力势下降引起的。我们的结果表明，二甲双胍对与表面感应和聚集相关的有鞭毛细菌的运动有显著影响。
