Department of Pharmaceutical Science, Beijing Institute of Radiation Medicine, Beijing, China.
Beijing Key Laboratory of Neuropsychopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
Biomed Chromatogr. 2022 Jul;36(7):e5379. doi: 10.1002/bmc.5379. Epub 2022 May 10.
Danggui Buxue Tang (DBT), a traditional Chinese medicine (TCM) formula for 'invigorating qi and enriching blood', has been reported to produce a good effect on chronic fatigue syndrome (CFS). However, the related mechanism remains largely undetermined. This study devised a metabolomics approach with GC-MS combined with pattern recognition to estimate the extent to which DBT alleviated CFS induced by food restriction and force swimming in rats. After 4 weeks of treatment, the endurance capability of rats was significantly better, and the motionless time was significantly shorter in the DBT group than in the CFS model group. Moreover, the activities of superoxide dismutase and glutathione peroxidase increased, whereas the levels of malondialdehyde, interleukin 6, and tumor necrosis factor alpha decreased in the DBT treatment group. Fifteen significantly changed metabolites were observed in the serum of rats with CFS, which was reversed markedly by DBT treatment. Metabolic pathway analysis showed that DBT could possibly alleviate CFS in rats by regulating the biosynthesis of phenylalanine, tyrosine, and tryptophan and the metabolism of glycine, serine, threonine, glycerolipid, glyoxylate, dicarboxylate, and tyrosine. It was observed that the metabolism of glycine, serine, and threonine was most closely related to the improvement in CFS by DBT treatment. This study showed that DBT could improve CFS effectively, and metabolomics was a powerful means to gain insights into the TCM formulas against CFS.
当归补血汤（DBT）是一种“补气养血”的中药方剂，据报道对慢性疲劳综合征（CFS）有很好的疗效。然而，相关机制在很大程度上仍未确定。本研究采用 GC-MS 结合模式识别的代谢组学方法，估算 DBT 缓解限制饮食和强迫游泳诱导的大鼠 CFS 的程度。经过 4 周的治疗，DBT 组大鼠的耐力能力明显提高，不动时间明显短于 CFS 模型组。此外，DBT 治疗组超氧化物歧化酶和谷胱甘肽过氧化物酶的活性增加，丙二醛、白细胞介素 6 和肿瘤坏死因子 α 的水平降低。在 CFS 大鼠的血清中观察到 15 种明显变化的代谢物，经 DBT 治疗后明显逆转。代谢途径分析表明，DBT 可能通过调节苯丙氨酸、酪氨酸和色氨酸的生物合成以及甘氨酸、丝氨酸、苏氨酸、甘油磷脂、乙醛酸、二羧酸和酪氨酸的代谢来缓解大鼠的 CFS。观察到甘氨酸、丝氨酸和苏氨酸的代谢与 DBT 治疗改善 CFS 密切相关。本研究表明，DBT 能有效改善 CFS，代谢组学是深入了解 CFS 中药方剂的有力手段。
