大肠杆菌RNA聚合酶在T7 DNA上引发的RNA合成的预引发流产。稳态动力学研究。
Primed abortive initiation of RNA synthesis by E. coli RNA polymerase on T7 DNA. Steady state kinetic studies.
作者信息
Smagowicz W J, Scheit K H
出版信息
Nucleic Acids Res. 1978 Jun;5(6):1919-32. doi: 10.1093/nar/5.6.1919.
Abstract
Ternary complexes of T7 DNA, RNA polymerase and the antibiotic rifampicin carry out the promoter specific abortive initiation when dinucleoside monophosphates were employed as primers. Primed abortive initiation, leading to synthesis of trinucleoside diphosphates, only occured with combinations of primers and substrates complementary to a promoter region of 8 base pairs centered around the origin of transcription. The steady state kinetics of three abortive initiations at T7 promoter A3 were studied in detail. The reactions appeared to be truly ordered. Affinity constants, maximal velocities and elementary step rate constants were thus obtained. The stimulation by dinucleoside monophosphate primers is brought about by positively effecting the function of the substrate site rather then by their higher affinity to the primer site of the transcriptional complex.
摘要
当使用二核苷单磷酸作为引物时，T7 DNA、RNA 聚合酶和抗生素利福平的三元复合物进行启动子特异性流产起始。引物介导的流产起始导致三核苷二磷酸的合成，仅在引物和底物与以转录起始点为中心的8个碱基对的启动子区域互补的组合中发生。详细研究了T7启动子A3处三次流产起始的稳态动力学。反应似乎是真正有序的。由此获得了亲和常数、最大速度和基本步骤速率常数。二核苷单磷酸引物的刺激是通过积极影响底物位点的功能，而不是通过它们对转录复合物引物位点的更高亲和力实现的。
相似文献
1
Primed abortive initiation of RNA synthesis by E. coli RNA polymerase on T7 DNA. Steady state kinetic studies.大肠杆菌RNA聚合酶在T7 DNA上引发的RNA合成的预引发流产。稳态动力学研究。
Nucleic Acids Res. 1978 Jun;5(6):1919-32. doi: 10.1093/nar/5.6.1919.
2
Steady state kinetic studies of initiation of RNA synthesis on T7 DNA in the presence of rifampicin.在利福平存在的情况下，对T7 DNA上RNA合成起始的稳态动力学研究。
Nucleic Acids Res. 1977 Nov;4(11):3863-76. doi: 10.1093/nar/4.11.3863.
3
Differential inhibition of abortive transcription initiation at different promoters catalysed by E. coli RNA polymerase. Effect of rifampicin on purine or pyramidine-initiated phosphodiester synthesis.大肠杆菌RNA聚合酶催化不同启动子处流产转录起始的差异抑制。利福平对嘌呤或嘧啶起始的磷酸二酯合成的影响。
FEBS Lett. 1992 Jul 13;306(1):46-50. doi: 10.1016/0014-5793(92)80834-4.
4
Interaction of RNA polymerase from Escherichia coli with DNA. Analysis of T7 DNA early-promoter sites.大肠杆菌RNA聚合酶与DNA的相互作用。T7 DNA早期启动子位点分析。
Eur J Biochem. 1975 Sep 15;57(2):569-78. doi: 10.1111/j.1432-1033.1975.tb02332.x.
5
A minimal mechanism for abortive initiation of transcription of T7 DNA.T7 DNA转录流产起始的一种最小机制。
Nucleic Acids Res. 1981 Nov 11;9(21):5845-54. doi: 10.1093/nar/9.21.5845.
6
An Escherichia coli RNA polymerase defective in transcription due to its overproduction of abortive initiation products.一种由于流产起始产物过量产生而在转录方面存在缺陷的大肠杆菌RNA聚合酶。
J Mol Biol. 1994 Feb 11;236(1):72-80. doi: 10.1006/jmbi.1994.1119.
7
Inhibition of T7 RNA polymerase: transcription initiation and transition from initiation to elongation are inhibited by T7 lysozyme via a ternary complex with RNA polymerase and promoter DNA.T7 RNA聚合酶的抑制：T7溶菌酶通过与RNA聚合酶和启动子DNA形成三元复合物，抑制转录起始以及从起始到延伸的转变。
Biochemistry. 1997 Nov 11;36(45):13954-62. doi: 10.1021/bi971432y.
8
[RNA polymerase of a rifampicin-resistant mutant of Escherichia coli has an altered selectivity to phage T7 DNA promoters].[大肠杆菌利福平抗性突变体的RNA聚合酶对噬菌体T7 DNA启动子的选择性发生改变]
Mol Biol (Mosk). 1988 Mar-Apr;22(2):384-92.
9
Stopped-flow kinetic analysis of the interaction of Escherichia coli RNA polymerase with the bacteriophage T7 A1 promoter.大肠杆菌RNA聚合酶与噬菌体T7 A1启动子相互作用的停流动力学分析。
J Mol Biol. 1998 Oct 23;283(2):353-70. doi: 10.1006/jmbi.1998.2101.
10
Phosphonate analogues of dinucleotides as substrates for DNA-dependent RNA polymerase from Escherichia coli in primed abortive initiation reaction.二核苷酸的膦酸酯类似物作为大肠杆菌DNA依赖性RNA聚合酶在引发的流产起始反应中的底物。
Int J Biol Macromol. 1989 Feb;11(1):33-8. doi: 10.1016/0141-8130(89)90037-8.
引用本文的文献
1
Promoter-sequence determinants and structural basis of primer-dependent transcription initiation in .启动子序列决定因素和. 中引物依赖性转录起始的结构基础。
Proc Natl Acad Sci U S A. 2021 Jul 6;118(27). doi: 10.1073/pnas.2106388118.
2
Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers.Förster 共振能量转移和蛋白质诱导的荧光增强作为协同的多尺度分子标尺。
Sci Rep. 2016 Sep 19;6:33257. doi: 10.1038/srep33257.
3
A Conserved Pattern of Primer-Dependent Transcription Initiation in Escherichia coli and Vibrio cholerae Revealed by 5' RNA-seq.5' RNA测序揭示大肠杆菌和霍乱弧菌中依赖引物的转录起始保守模式
PLoS Genet. 2015 Jul 1;11(7):e1005348. doi: 10.1371/journal.pgen.1005348. eCollection 2015 Jul.
4
The transcription bubble of the RNA polymerase-promoter open complex exhibits conformational heterogeneity and millisecond-scale dynamics: implications for transcription start-site selection.RNA 聚合酶-启动子开放复合物的转录泡表现出构象异质性和毫秒级动力学：对转录起始位点选择的影响。
J Mol Biol. 2013 Mar 11;425(5):875-85. doi: 10.1016/j.jmb.2012.12.015. Epub 2012 Dec 28.
5
A new way to start: nanoRNA-mediated priming of transcription initiation.一种新的起始方式：纳米RNA介导的转录起始引发
Transcription. 2012 Nov-Dec;3(6):300-4. doi: 10.4161/trns.21903. Epub 2012 Nov 1.
6
NanoRNAs: a class of small RNAs that can prime transcription initiation in bacteria.纳米 RNA：一类能在细菌中引发转录起始的小 RNA。
J Mol Biol. 2011 Oct 7;412(5):772-81. doi: 10.1016/j.jmb.2011.06.015. Epub 2011 Jun 16.
7
NanoRNAs prime transcription initiation in vivo.小 RNA 可在体内引发转录起始。
Mol Cell. 2011 Jun 24;42(6):817-25. doi: 10.1016/j.molcel.2011.06.005.
8
RNA polymerase: linear competitive inhibition by bis-(3' to 5')-cyclic dinucleotides,NpNp.RNA聚合酶：双（3'至5'）环二核苷酸NpNp的线性竞争性抑制作用
Nucleic Acids Res. 1982 Sep 25;10(18):5637-47. doi: 10.1093/nar/10.18.5637.
9
A minimal mechanism for abortive initiation of transcription of T7 DNA.T7 DNA转录流产起始的一种最小机制。
Nucleic Acids Res. 1981 Nov 11;9(21):5845-54. doi: 10.1093/nar/9.21.5845.
10
The properties of ATP-analogs in initiation of RNA synthesis catalyzed by RNA polymerase from E coli.ATP类似物在大肠杆菌RNA聚合酶催化的RNA合成起始过程中的特性。
Nucleic Acids Res. 1981 May 25;9(10):2397-410. doi: 10.1093/nar/9.10.2397.
本文引用的文献
1
A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.一种从大肠杆菌大规模制备高纯度依赖DNA的RNA聚合酶的新方法。
Hoppe Seylers Z Physiol Chem. 1970 Feb;351(2):221-4. doi: 10.1515/bchm2.1970.351.1.221.
2
3
Transcription of the early region of bacteriophage T7: selective initiation with dinucleotides.噬菌体T7早期区域的转录：二核苷酸的选择性起始
J Mol Biol. 1973 Jun 25;77(2):255-77. doi: 10.1016/0022-2836(73)90335-5.
4
Studies of the binding of Escherichia coli RNA polymerase to DNA. V. T7 RNA chain initiation by enzyme-DNA complexes.大肠杆菌RNA聚合酶与DNA结合的研究。V. 酶-DNA复合物引发的T7 RNA链起始
J Mol Biol. 1972 Sep 28;70(2):221-37. doi: 10.1016/0022-2836(72)90535-9.
5
Rapid isolation of highly active RNA polymerase from Escherichia coli and its subunits by matrix-bound heparin.通过基质结合肝素从大肠杆菌及其亚基中快速分离高活性RNA聚合酶
Eur J Biochem. 1975 Dec 1;60(1):51-5. doi: 10.1111/j.1432-1033.1975.tb20974.x.
6
Interaction of RNA polymerase from Escherichia coli with DNA. Analysis of T7 DNA early-promoter sites.大肠杆菌RNA聚合酶与DNA的相互作用。T7 DNA早期启动子位点分析。
Eur J Biochem. 1975 Sep 15;57(2):569-78. doi: 10.1111/j.1432-1033.1975.tb02332.x.
7
Interaction of RNA polymerase from Escherichia coli with DNA. Effect of temperature and ionic strength on selection of T7 DNA early promoters.大肠杆菌RNA聚合酶与DNA的相互作用。温度和离子强度对T7 DNA早期启动子选择的影响。
Eur J Biochem. 1976 Jun 1;65(2):387-93. doi: 10.1111/j.1432-1033.1976.tb10352.x.
8
An expanded transcriptional map of T7 bacteriophage. Reading of minor T7 promoter sites in vitro by Escherichia coli RNA polymerase.T7噬菌体的扩展转录图谱。大肠杆菌RNA聚合酶在体外对次要T7启动子位点的读取。
J Mol Biol. 1977 Jun 5;112(4):577-601. doi: 10.1016/s0022-2836(77)80165-4.
9
Steady state kinetic studies of initiation of RNA synthesis on T7 DNA in the presence of rifampicin.在利福平存在的情况下，对T7 DNA上RNA合成起始的稳态动力学研究。
Nucleic Acids Res. 1977 Nov;4(11):3863-76. doi: 10.1093/nar/4.11.3863.
Zotero 插件