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RNA聚合酶：双（3'至5'）环二核苷酸NpNp的线性竞争性抑制作用

RNA polymerase: linear competitive inhibition by bis-(3' to 5')-cyclic dinucleotides,NpNp.

作者信息

Hsu C Y, Dennis D

出版信息

Nucleic Acids Res. 1982 Sep 25;10(18):5637-47. doi: 10.1093/nar/10.18.5637.

DOI:10.1093/nar/10.18.5637
PMID:7145712
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320912/
Abstract

We have investigated the possible role of the bis-(3' to 5')-cyclic dinucleotides UpUp and ApUp as kinetic inhibitors of the DNA dependent RNA polymerase enzyme of E. coli, using T7 delta D111 deletion mutant DNA and several synthetic DNA polymers as templates. We have established that UpUp is a linear competitive inhibitor of the initiation phase of the polymerization (Ki = 28 microM using T7 delta D111 DNA as a template), but that it has no effect when added during the elongation phase. The compound ApUp is an inhibitor of the reaction only when poly(dA-T).poly(dA-T) is used as a template, and UpUp is an inhibitor of the reaction when poly(dA).poly(dT) was employed as the DNA template.

摘要

我们使用T7 δD111缺失突变体DNA和几种合成DNA聚合物作为模板，研究了双（3'至5'）环二核苷酸UpUp和ApUp作为大肠杆菌DNA依赖性RNA聚合酶的动力学抑制剂的可能作用。我们已经确定，UpUp是聚合起始阶段的线性竞争性抑制剂（使用T7 δD111 DNA作为模板时，Ki = 28 μM），但在延伸阶段添加时没有作用。化合物ApUp仅在使用聚（dA-T）·聚（dA-T）作为模板时是反应抑制剂，而当使用聚（dA）·聚（dT）作为DNA模板时，UpUp是反应抑制剂。

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Primed abortive initiation of RNA synthesis by E. coli RNA polymerase on T7 DNA. Steady state kinetic studies.大肠杆菌RNA聚合酶在T7 DNA上引发的RNA合成的预引发流产。稳态动力学研究。
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