• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Steady state kinetic studies of initiation of RNA synthesis on T7 DNA in the presence of rifampicin.在利福平存在的情况下，对T7 DNA上RNA合成起始的稳态动力学研究。
Nucleic Acids Res. 1977 Nov;4(11):3863-76. doi: 10.1093/nar/4.11.3863.
2
Substrate selection by RNA polymerase from E. coli. The role of ribose and 5'-triphosphate fragments, and nucleotides interaction.大肠杆菌RNA聚合酶的底物选择。核糖和5'-三磷酸片段的作用以及核苷酸间的相互作用。
Acta Biochim Pol. 1985;32(4):329-49.
3
Stopped-flow kinetic analysis of the interaction of Escherichia coli RNA polymerase with the bacteriophage T7 A1 promoter.大肠杆菌RNA聚合酶与噬菌体T7 A1启动子相互作用的停流动力学分析。
J Mol Biol. 1998 Oct 23;283(2):353-70. doi: 10.1006/jmbi.1998.2101.
4
Primed abortive initiation of RNA synthesis by E. coli RNA polymerase on T7 DNA. Steady state kinetic studies.大肠杆菌RNA聚合酶在T7 DNA上引发的RNA合成的预引发流产。稳态动力学研究。
Nucleic Acids Res. 1978 Jun;5(6):1919-32. doi: 10.1093/nar/5.6.1919.
5
UTP allosterically regulates transcription by Escherichia coli RNA polymerase from the bacteriophage T7 A1 promoter.尿苷三磷酸（UTP）对来自噬菌体T7 A1启动子的大肠杆菌RNA聚合酶的转录具有变构调节作用。
J Mol Biol. 2002 Apr 26;318(2):305-20. doi: 10.1016/S0022-2836(02)00042-6.
6
Studies of the binding of Escherichia coli RNA polymerase to DNA. V. T7 RNA chain initiation by enzyme-DNA complexes.大肠杆菌RNA聚合酶与DNA结合的研究。V. 酶-DNA复合物引发的T7 RNA链起始
J Mol Biol. 1972 Sep 28;70(2):221-37. doi: 10.1016/0022-2836(72)90535-9.
7
The properties of ATP-analogs in initiation of RNA synthesis catalyzed by RNA polymerase from E coli.ATP类似物在大肠杆菌RNA聚合酶催化的RNA合成起始过程中的特性。
Nucleic Acids Res. 1981 May 25;9(10):2397-410. doi: 10.1093/nar/9.10.2397.
8
[Kinetics of inhibition by 8-oxy-GTP and 8-bromo-GTP of Escherichia coli RNA-polymerase synthesis of pppApU dinucleotide on the promotor a1 of phage T7deltaD111 DNA in a limited set of substrates].[在一组有限的底物中，8-氧代-GTP和8-溴代-GTP对噬菌体T7δD111 DNA启动子a1上大肠杆菌RNA聚合酶合成三磷酸腺苷-尿苷二核苷酸的抑制动力学]
Mol Biol (Mosk). 1989 May-Jun;23(3):822-9.
9
Studies of the binding of Escherichia coli RNA polymerase to DNA. IV. The effect of rifampicin on binding and on RNA chain initiation.大肠杆菌RNA聚合酶与DNA结合的研究。IV. 利福平对结合及RNA链起始的影响。
J Mol Biol. 1972 Sep 28;70(2):209-20. doi: 10.1016/0022-2836(72)90534-7.
10
Kinetic analysis of ribonucleic acid chain initiation by Escherichia coli Ribonucleic acid polymerase bound to DNA.大肠杆菌核糖核酸聚合酶与DNA结合时核糖核酸链起始的动力学分析。
J Biol Chem. 1975 Dec 10;250(23):9112-20.

引用本文的文献

1
A minimal mechanism for abortive initiation of transcription of T7 DNA.T7 DNA转录流产起始的一种最小机制。
Nucleic Acids Res. 1981 Nov 11;9(21):5845-54. doi: 10.1093/nar/9.21.5845.
2
The properties of ATP-analogs in initiation of RNA synthesis catalyzed by RNA polymerase from E coli.ATP类似物在大肠杆菌RNA聚合酶催化的RNA合成起始过程中的特性。
Nucleic Acids Res. 1981 May 25;9(10):2397-410. doi: 10.1093/nar/9.10.2397.
3
Mechanistic aspects of promoter binding and chain initiation by RNA polymerase.RNA聚合酶与启动子结合及链起始的机制方面。
Mol Cell Biochem. 1982 Sep 17;47(3):129-49. doi: 10.1007/BF00229597.
4
Isolation, characterization and possible mode of action of antiseminalplasmin, a new protein that inhibits the antimicrobial activity of seminalplasmin.抗精浆纤溶酶的分离、特性鉴定及其可能的作用方式，抗精浆纤溶酶是一种抑制精浆纤溶酶抗菌活性的新蛋白质。
Biochem J. 1985 Apr 15;227(2):609-19. doi: 10.1042/bj2270609.
5
Primed abortive initiation of RNA synthesis by E. coli RNA polymerase on T7 DNA. Steady state kinetic studies.大肠杆菌RNA聚合酶在T7 DNA上引发的RNA合成的预引发流产。稳态动力学研究。
Nucleic Acids Res. 1978 Jun;5(6):1919-32. doi: 10.1093/nar/5.6.1919.

本文引用的文献

1
Studies on the kinetics of ribonucleic acid chain initiation and elongation.核糖核酸链起始与延伸动力学研究。
Biochemistry. 1970 Jun 9;9(12):2520-5. doi: 10.1021/bi00814a019.
2
A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.一种从大肠杆菌大规模制备高纯度依赖DNA的RNA聚合酶的新方法。
Hoppe Seylers Z Physiol Chem. 1970 Feb;351(2):221-4. doi: 10.1515/bchm2.1970.351.1.221.
3
Ribonucleic acid chain elongation by Escherichia coli ribonucleic acid polymerase. I. Isolation of ternary complexes and the kinetics of elongation.大肠杆菌核糖核酸聚合酶催化的核糖核酸链延伸。I. 三元复合物的分离及延伸动力学
J Biol Chem. 1974 Oct 25;249(20):6675-83.
4
Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. I. An assay for the rate and extent of ribonucleic acid chain initiation.大肠杆菌核糖核酸聚合酶与T7脱氧核糖核酸结合时核糖核酸链起始的研究。I. 核糖核酸链起始速率和程度的测定方法
J Biol Chem. 1974 May 25;249(10):2995-3001.
5
Studies on polynucleotides. CXX. On the transcription of a synthetic 29-unit long deoxyribopolynucleotide.多核苷酸研究。CXX。关于一个29个单位长的脱氧核糖多核苷酸的转录。
J Biol Chem. 1972 Oct 10;247(19):6157-66.
6
Rapid isolation of highly active RNA polymerase from Escherichia coli and its subunits by matrix-bound heparin.通过基质结合肝素从大肠杆菌及其亚基中快速分离高活性RNA聚合酶
Eur J Biochem. 1975 Dec 1;60(1):51-5. doi: 10.1111/j.1432-1033.1975.tb20974.x.
7
Kinetic analysis of ribonucleic acid chain initiation by Escherichia coli Ribonucleic acid polymerase bound to DNA.大肠杆菌核糖核酸聚合酶与DNA结合时核糖核酸链起始的动力学分析。
J Biol Chem. 1975 Dec 10;250(23):9112-20.
8
Characterization of the nucleoside triphosphate phosphohydrolase (ATPase) activity of RNA synthesi termination factor p. I. Enzymatic properties and effects of inhibitors.RNA合成终止因子ρ的核苷三磷酸磷酸水解酶（ATP酶）活性的表征。I. 酶学性质及抑制剂的作用
J Biol Chem. 1977 Feb 25;252(4):1375-80.

在利福平存在的情况下，对T7 DNA上RNA合成起始的稳态动力学研究。

Steady state kinetic studies of initiation of RNA synthesis on T7 DNA in the presence of rifampicin.

作者信息

Smagowicz J W, Scheit K H

出版信息

Nucleic Acids Res. 1977 Nov;4(11):3863-76. doi: 10.1093/nar/4.11.3863.

DOI:10.1093/nar/4.11.3863
PMID:593891
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343206/
Abstract

The steady state kinetics of initiation of T7 DNA transcription by RNA polymerase holo enzyme from E. coli in the presence of rifampicin and the two substrates ATP and UTP were studied. Under these conditions, the enzyme catalyzes exclusively the promotor specific synthesis of pppApU. The kinetic data are in agreement with the mechanism of a truly ordered reaction. Binding of the initiating nucleotide ATP to the transcriptional complex occurs prior to the binding of the substrate UTP. Release of pppApU is most probably the rate limitinig step. Km constants were found to be 0.6 mM for ATP and 0.31 mM for UTP, respectively. The substrate inhibition pattern indicated that the substrate site exhibits a finite affinity for incorrect nucleoside triphosphate (Ki = 2.3 mM). A similar non specific binding to the 3-OH site could not be demonstrated.

摘要

研究了在利福平以及两种底物ATP和UTP存在的情况下，大肠杆菌RNA聚合酶全酶启动T7 DNA转录的稳态动力学。在这些条件下，该酶仅催化pppApU的启动子特异性合成。动力学数据与真正有序反应的机制一致。起始核苷酸ATP与转录复合物的结合发生在底物UTP结合之前。pppApU的释放很可能是限速步骤。发现ATP的Km常数分别为0.6 mM，UTP的Km常数为0.31 mM。底物抑制模式表明底物位点对错误的核苷三磷酸具有有限的亲和力（Ki = 2.3 mM）。未证明与3'-OH位点有类似的非特异性结合。