一种针对成人人脑的新型高尔基染色法。
A new Golgi method for adult human brains.
作者信息
Armstrong E, Parker B
出版信息
J Neurosci Methods. 1986 Sep;17(4):247-54. doi: 10.1016/0165-0270(86)90124-x.
PMID:3537537
Abstract
A new fast and reliable Golgi method to impregnate neurons in adult human brains which have been routinely fixed in neutral buffered formalin is described. In addition to potassium dichromate and sucrose, this technique uses microwave energy and hydrogen peroxide. Dendrites and perikarya of cortical pyramidal neurons and large subcortical multipolar neurons are the neuronal structures most commonly impregnated by this method.
摘要
本文描述了一种全新的快速且可靠的高尔基方法，用于浸染常规固定于中性缓冲福尔马林中的成人大脑神经元。除重铬酸钾和蔗糖外，该技术还使用了微波能量和过氧化氢。皮质锥体细胞和大的皮质下多极神经元的树突和胞体是最常通过此方法浸染的神经元结构。
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