Marani E, Guldemond J M, Adriolo P J, Boon M E, Kok L P
Laboratory for Anatomy and Embryology, University of Leiden, The Netherlands.
Histochem J. 1987 Dec;19(12):658-64. doi: 10.1007/BF01676172.
Application of microwaves in histochemistry and cytochemistry generally speeds up the technique. Microwaves stimulate diffusion into the tissue and influence the proteins and membrane of the cell. Silver impregnation techniques for the brain, such as the fast Rio-Hortega or the slow Golgi-Cox technique, normally require a minimum time period of 7 days and 20 days respectively. Using microwaves, the Rio-Hortega technique can be completed within 24 h. In sections prepared from mature brains, good silver impregnation of cell bodies, of axons and their terminals, and of dendrites and their spines are obtained. An explanation is given as to why the method cannot be further reduced in time. To our knowledge, this is the first report of the application of microwave irradiation for colouring pieces of tissue.
微波在组织化学和细胞化学中的应用通常会加快技术进程。微波刺激物质扩散进入组织，并影响细胞的蛋白质和细胞膜。用于大脑的银浸染技术，如快速的里约-奥尔特加技术或缓慢的高尔基-考克斯技术，通常分别需要至少7天和20天的时间。使用微波，里约-奥尔特加技术可以在24小时内完成。在从成熟大脑制备的切片中，可以获得细胞体、轴突及其终末、树突及其棘的良好银浸染效果。文中给出了该方法为何不能进一步缩短时间的解释。据我们所知，这是关于微波辐射用于组织染色的首次报道。
