Department of Chemistry, Fudan University, Shanghai 200438, China.
Anal Chem. 2022 Apr 19;94(15):5723-5728. doi: 10.1021/acs.analchem.2c00231. Epub 2022 Apr 4.
Cancer-cell-derived exosomes are regarded as noninvasive biomarkers for early cancer diagnosis because of their critical roles in intercellular communication and molecular exchange. A robust aptamer-initiated catalytic hairpin assembly (AICHA) fluorescence assay is proposed for universal, sensitive detection of cancer-derived exosomes. The AICHA was verified with the specific detection of MCF-7 cell-derived exosomes with a wide calibration range of 8.4 particles/μL to 8.4 × 10 particles/μL and a low detection limit (LOD) of 0.5 particles/μL. The universality of the AICHA method was verified for PANC-1 cell-derived exosomes, the LOD of which was determined to be 0.1 particles/μL. The performances in serum samples were detected with a recovery rate range of 95.45-106.2%, which demonstrates its significant potential for protein biomarker analysis and cancer diagnosis.
癌细胞衍生的外泌体因其在细胞间通讯和分子交换中的关键作用而被视为非侵入性的早期癌症诊断生物标志物。提出了一种强大的适体引发的催化发夹组装（AICHA）荧光分析方法，用于普遍、灵敏地检测癌症衍生的外泌体。AICHA 通过特异性检测 MCF-7 细胞衍生的外泌体得到验证，其校准范围很宽，为 8.4 个/μL 至 8.4×10 个/μL，检测限（LOD）低至 0.5 个/μL。AICHA 方法的通用性已通过 PANC-1 细胞衍生的外泌体得到验证，其 LOD 确定为 0.1 个/μL。通过血清样品中的性能检测，回收率范围为 95.45-106.2%，这表明其在蛋白质生物标志物分析和癌症诊断方面具有重要的应用潜力。
