Department of Stomatology, Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China.
School of Stomatology, Qingdao University, Qingdao 266003, China.
ACS Appl Mater Interfaces. 2022 Apr 13;14(14):16006-16017. doi: 10.1021/acsami.2c00877. Epub 2022 Apr 4.
Soft-tissue trauma emergency caused by natural disasters and traffic accidents is highly prevalent, which can result in massive bleeding, pathogen infection, and even death. Although numerous tissue adhesives can bind to tissue surfaces and cover wounds, most of them still have several deficiencies, including long gelation time, poor adhesive strength, and anti-infection, making them inappropriate for use as first-aid bandages. Herein, injectable and self-healing four-arm-PEG-CHO/polyethyleneimine (PEI) tissue adhesives as liquid first-aid supplies are developed via the dynamic Schiff base reaction for trauma emergency. It is found that the prepared hydrogel adhesives exhibit short and controlled gelation time (9∼88 s), strong adhesive strength, and excellent antibacterial ability. Their hemostatic and antimicrobial performances can be tailored by the mass ratio of four-arm-PEG-CHO/PEI. Moreover, in vitro biological assays display that the developed tissue adhesives possess satisfactory cyto/hemocompatibility. Importantly, in vivo the designed adhesives show fast hemostatic capacity and excellent anti-infection as compared to commercial Prontosan gel. Thus, this work indicates that the four-arm-PEG-CHO/PEI first-aid tissue adhesives display great potential for wound emergency management.
自然灾害和交通事故导致的软组织创伤急诊非常普遍，可导致大量出血、病原体感染，甚至死亡。尽管有许多组织粘合剂可以与组织表面结合并覆盖伤口，但它们大多仍存在几个缺点，包括凝胶时间长、粘合强度差和抗感染能力差，因此不适合用作急救绷带。在此，通过动态席夫碱反应开发了可注射和自修复的四臂-PEG-CHO/聚乙烯亚胺（PEI）组织粘合剂，作为液体急救用品，用于创伤急诊。结果表明，所制备的水凝胶粘合剂具有较短且可控的凝胶时间（9∼88 s）、较强的粘合强度和优异的抗菌能力。其止血和抗菌性能可以通过四臂-PEG-CHO/PEI 的质量比进行调整。此外，体外生物学试验表明，所开发的组织粘合剂具有良好的细胞/血液相容性。重要的是，与商业的 Prontosan 凝胶相比，体内实验表明，设计的粘合剂具有快速止血能力和优异的抗感染能力。因此，这项工作表明，四臂-PEG-CHO/PEI 急救组织粘合剂在伤口急救管理方面具有巨大的潜力。
