Rangachari K, Dluzewski A, Wilson R J, Gratzer W B
Nature. 1986;324(6095):364-5. doi: 10.1038/324364a0.
It has been shown that the entry of the malaria parasite into the red blood cell requires the presence of ATP in the host cell cytoplasm. In red blood cell ghosts that contain no ATP the receptor on the extracellular surface remains in place and parasites will bind to the membrane, but will not enter. ATP is thus necessary for one of the steps in the invasion sequence that follows recognition and attachment. The process of entry appears to involve the active participation of the host cell membrane cytoskeleton. We have suggested that the function of the intracellular ATP may be to regulate phosphorylation of the cytoskeleton. We now present evidence that the activity of the membrane-associated cyclic AMP-independent kinase of the red blood cell is inseparable from invasion; the active substrate may be spectrin.
已经表明，疟原虫进入红细胞需要宿主细胞质中存在ATP。在不含ATP的红细胞血影中，细胞外表面的受体保持原位，寄生虫会与膜结合，但不会进入。因此，ATP是识别和附着后入侵序列中一个步骤所必需的。进入过程似乎涉及宿主细胞膜细胞骨架的积极参与。我们曾提出，细胞内ATP的功能可能是调节细胞骨架的磷酸化。我们现在提供证据表明，红细胞膜相关的非环磷酸腺苷依赖性激酶的活性与入侵密不可分；活性底物可能是血影蛋白。
