Liu Fei, Yang Yuemeng, Wan Xizi, Gao Hongxiao, Wang Yulu, Lu Jingwei, Xu Li-Ping, Wang Shutao
Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
CAS Key Laboratory of Bio-inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
ACS Nano. 2022 Apr 26;16(4):6266-6273. doi: 10.1021/acsnano.2c00157. Epub 2022 Apr 6.
Fluorescent biosensors have been widely applied in DNA detection because of their reliability and reproducibility. However, low kinetics in DNA hybridization often brings out long test terms, thus restricting their practical use. Here, we demonstrate unexpected fast DNA fluorescence detection on the confined surface of hydrogel particles. When the pore size and surface charge of hydrogel particles are tailored, DNA molecules can be confined in the outer water layer of hydrogel particles. We fabricated a fluorescence-on DNA sensor based on the hydrogel particle array by utilizing the fluorescence quenching property of graphene oxide and its different adsorption behaviors toward single-strand DNA or double-strand DNA. Benefiting from the confinement effect of hydrogel particle surface and the enrichment effect of water evaporation, the DNA-recognition time was descreased significantly from 3000 s to less than 10 s under the target concentration of 400 nM. Moreover, rapid detection can be achieved at concentrations between 50 and 400 nM. The study provides another insight to fabricate fast biosensors and shows great potential in DNA diagnostics, gene analysis, and liquid biopsy.
荧光生物传感器因其可靠性和可重复性已被广泛应用于DNA检测。然而，DNA杂交过程中的低动力学常常导致检测时间过长，从而限制了它们的实际应用。在此，我们展示了在水凝胶颗粒受限表面上实现意外快速的DNA荧光检测。当对水凝胶颗粒的孔径和表面电荷进行调整时，DNA分子可以被限制在水凝胶颗粒的外层水层中。我们利用氧化石墨烯的荧光猝灭特性及其对单链DNA或双链DNA的不同吸附行为，基于水凝胶颗粒阵列构建了一种开启型DNA传感器。受益于水凝胶颗粒表面的限制效应和水蒸发的富集效应，在目标浓度为400 nM时，DNA识别时间从3000秒显著缩短至不到10秒。此外，在50至400 nM的浓度范围内均可实现快速检测。该研究为制造快速生物传感器提供了另一种思路，并在DNA诊断、基因分析和液体活检中显示出巨大潜力。
