Kume Eni, Almohammadi Ghadeer, Duleba Dominik, M Alotaibi Aeshah Farhan, Gan Rongcheng, Mamaeva Kseniia, Bradley A Louise, Johnson Robert P, Rice James H
School of Physics, University College Dublin, Belfield, Dublin 4, D04 V1W8, Ireland.
School of Chemistry, University College Dublin, Belfield, Dublin 4, D04 V1W8, Ireland.
ACS Appl Mater Interfaces. 2025 May 14;17(19):28881-28893. doi: 10.1021/acsami.5c03428. Epub 2025 May 5.
Fluorescence based detection is applied across various fields, including medical diagnostics and environmental sensing. A key challenge in these technologies lies in optimizing sensitivity through enhancement of the fluorescence signal. In this study, we demonstrate that combining piezoelectric and plasmonic processes increases the fluorescence yield. Piezoelectric poly(vinylidene fluoride--hexafluoropropylene) (PVDF-HFP), is utilized as an external electric field modulator to produce a reliable and reproducible fluorescence enhancement of InP/ZnS quantum dots approaching the single nanoparticle level. The relationship between the applied force and the fluorescence response is both experimentally quantified and theoretically modeled and the dependence of the fluorescence enhancement on the excitation wavelength and on the PVDF-HFP substrate topography is elucidated. Furthermore, fluorescence enhancement by a magnitude of order for a DNA hybridization assay on the gold-coated PVDF-HFP substrate is demonstrated, highlighting the practical applicability of this approach in biosensing.
基于荧光的检测技术已广泛应用于各个领域，包括医学诊断和环境传感。这些技术面临的一个关键挑战是通过增强荧光信号来优化灵敏度。在本研究中，我们证明了将压电和等离子体过程相结合可提高荧光产率。压电聚偏二氟乙烯-六氟丙烯（PVDF-HFP）被用作外部电场调制器，以实现可靠且可重复的InP/ZnS量子点荧光增强，接近单个纳米颗粒水平。实验定量了施加力与荧光响应之间的关系，并进行了理论建模，阐明了荧光增强对激发波长和PVDF-HFP基底形貌的依赖性。此外，还展示了在金涂层PVDF-HFP基底上进行DNA杂交检测时荧光增强了一个数量级，突出了该方法在生物传感中的实际应用价值。
