Lee Pureun-Haneul, Choi SeonMuk, An MinHyeok, Jang An-Soo
Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
Clin Transl Allergy. 2022 Mar 31;12(4):e12136. doi: 10.1002/clt2.12136. eCollection 2022 Apr.
Asthma is diagnosed based on a history of the characteristic symptoms and evidence of expiratory airflow limitation. However, asthma diagnosis using the existing tests is associated with a risk of coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 spread. In this study, we developed a quantitative real-time polymerase chain reaction (qRT-PCR)-based asthma diagnosis tool.
We detected nectin-4 in the plasma of patients with asthma using qRT-PCR, explored the relationship of clinical variables.
quantitative real-time polymerase chain reaction revealed that plasma nectin-4 mRNA levels were higher in asthmatics than controls. These results correlated with lung function.
Those results suggest that qRT-PCR for nectin-4 may aid asthma diagnosis and monitoring.
哮喘是根据特征性症状病史和呼气气流受限证据来诊断的。然而，使用现有检测方法进行哮喘诊断存在因严重急性呼吸综合征冠状病毒2传播导致的2019冠状病毒病风险。在本研究中，我们开发了一种基于定量实时聚合酶链反应（qRT-PCR）的哮喘诊断工具。
我们使用qRT-PCR检测哮喘患者血浆中的nectin-4，探讨临床变量之间的关系。
定量实时聚合酶链反应显示，哮喘患者血浆中nectin-4 mRNA水平高于对照组。这些结果与肺功能相关。
这些结果表明，针对nectin-4的qRT-PCR可能有助于哮喘的诊断和监测。
