Gonçalves da Costa Sousa Mauricio, Conceição de Almeida Gabriela, Martins Mota Danilo César, Andrade da Costa Rosiane, Dias Simoni Campos, Limberger Samuel Nunes, Ko Frank, Lin Li Ting, Haney Evan F, Etayash Hashem, Baquir Beverlie, Trimble Michael J, Shen Ya, Su Zheng, Haapasalo Markus, Pletzer Daniel, Chaves de Souza Letícia, Schuindt Teixeira Gláucia, Silva Renato M, Hancock Robert E W, Franco Octavio Luiz, Berto Rezende Taia Maria
Post-Graduation Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, Brazil.
Dentistry Course, Catholic University of Brasília, Brasília, Brazil.
Bioact Mater. 2022 Feb 1;16:173-186. doi: 10.1016/j.bioactmat.2022.01.027. eCollection 2022 Oct.
Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry. Here, different scaffolds were tested for use during the pulp revascularization process, including poly(vinyl alcohol)-PVA hydrogels or resins, collagen hydrogels and poly(vinyl alcohol) PVA/Chitosan (PVA/CS) nanofibers. Based on time to degradation (21 days), nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002 (each at 50 mg/g). Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against and a multispecies oral biofilm, besides anti-inflammatory activities. The subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation, when compared to empty teeth fragments. Thus, we designed a strong antimicrobial, immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures.
具有宿主防御肽的多功能支架专为再生性牙髓病学设计，是牙科领域理想的纳米生物技术工具。在此，对不同的支架在牙髓血运重建过程中的应用进行了测试，包括聚乙烯醇（PVA）水凝胶或树脂、胶原蛋白水凝胶以及聚乙烯醇（PVA）/壳聚糖（PVA/CS）纳米纤维。基于降解时间（21天），选择纳米纤维与环丙沙星和IDR-1002（各50毫克/克）结合。含有环丙沙星和IDR-1002的纳米纤维除了具有抗炎活性外，还对单一菌种和多菌种口腔生物膜具有抗生物膜活性。与空牙碎片相比，填充纳米纤维的牙碎片对皮下组织的反应显示出牙髓样组织的形成。因此，我们为牙髓血运重建和再生程序设计了一种强大的抗菌、免疫调节和再生候选物。
