Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki (A.U.Th), GR-54124, Thessaloniki, Greece.
Centre National de la Recherche Scientifique, Institute of Movement Sciences, Aix Marseille University, Marseille, France.
Stem Cell Rev Rep. 2021 Jun;17(3):785-802. doi: 10.1007/s12015-020-10069-8. Epub 2020 Nov 3.
Experimental procedures have been used to monitor cellular responses at the dentin/pulp interface. Aiming to divert from in vivo studies and oversimplified two-dimensional assays, three-dimensional (3D) models have been developed. This review provides an overview of existing literature, regarding 3D in vitro dentin/pulp reconstruction.
MATERIAL & METHODS: PubMed, Scopus, Cochrane Library and Web of Science- were systematically searched for attributes between 1998 and 2020. The search focused on articles on the development of three-dimensional tools for the reconstruction of a dentin/pulp complex under in vitro conditions, which were then screened and qualitatively assessed. Article grouping according to mode of implementation, resulted in five categories: the customised cell perfusion chamber (CPC) (n = 8), the tooth bud model (TBM) (n = 3), the 3D dentin/pulp complex manufactured by tissue engineering (DPC) (n = 6), the entire tooth culture (ETC) (n = 4) and the tooth slice culture model (TSC) (n = 5).
A total of 26 publications, applying nine and eight substances for pulp and dentin representation respectively, were included. Natural materials and dentin components were the most widely utilized. The most diverse category was the DPC, while the CPC group was the test with the highest longevity. The most consistent categories were the ETC and TSC models, while the TBM presented as the most complete de novo approach.
All studies presented with experimental protocols with potential upgrades. Solving the limitations of each category will provide a complete in vitro testing and monitoring tool of dental responses to exogenous inputs.
The 3D dentin/pulp complexes are valid supplementary tools for in vivo studies and clinical testing. Graphical Abstract.
实验程序已被用于监测牙本质/牙髓界面的细胞反应。为了避免体内研究和过于简化的二维分析，已经开发了三维（3D）模型。本综述概述了现有的文献，涉及 3D 体外牙本质/牙髓重建。
系统地检索了 1998 年至 2020 年期间的 PubMed、Scopus、Cochrane 图书馆和 Web of Science 中的文献，重点是关于在体外条件下重建牙本质/牙髓复合体的三维工具的发展的文章，然后对这些文章进行筛选和定性评估。根据实施方式对文章进行分组，分为五类：定制细胞灌注室（CPC）（n=8）、牙蕾模型（TBM）（n=3）、组织工程制造的 3D 牙本质/牙髓复合体（DPC）（n=6）、整个牙齿培养（ETC）（n=4）和牙切片培养模型（TSC）（n=5）。
共纳入 26 篇文献，分别应用了 9 种和 8 种物质来代表牙髓和牙本质。天然材料和牙本质成分是最广泛使用的。最具多样性的类别是 DPC，而 CPC 组是寿命最长的测试。最一致的类别是 ETC 和 TSC 模型，而 TBM 则是最完整的从头开始方法。
所有研究都提出了具有潜在升级的实验方案。解决每个类别的局限性将提供一种完整的体外测试和监测工具，用于监测牙齿对外源性刺激的反应。
3D 牙本质/牙髓复合体是体内研究和临床测试的有效补充工具。
