Hossenbaccus Lubnaa, Linton Sophia, Thiele Jenny, Steacy Lisa, Walker Terry, Malone Crystal, Ellis Anne K
Department of Medicine, Queen's University, Kingston, ON, Canada.
Allergy Research Unit, Kingston Health Sciences Centre-KGH Site, Kingston, ON, Canada.
Front Allergy. 2022 Jan 7;2:807208. doi: 10.3389/falgy.2021.807208. eCollection 2021.
Allergic rhinitis (AR) is an inflammatory disease of the nasal mucosa that can be modeled using Controlled Allergen Exposure Facilities (CACF). Recently, we clinically validated the house dust mite (HDM) Environmental Exposure Unit (EEU) facility. In the current study, we aimed to assess biological responses in the blood following HDM exposure in the HDM-EEU. Fifty-five participants passed a screening visit, where they provided consent and completed a skin prick test (SPT), then attended a modest or higher HDM exposure session. Baseline and post-exposure blood samples were collected. Complete blood counts with differentials were measured, and isolated serum was used to determine - and -specific IgE (sIgE) and cytokine concentrations (IL-4, IL-5, IL-6, IL-10, IL-13, TNF-α). HDM-allergic participants had significantly greater SPT wheal sizes than healthy controls. sIgE concentrations were significantly greater in allergic participants, with a strong correlation between and . Serum eosinophil counts were significantly decreased post-exposure for allergic participants. White blood cell, neutrophil, and lymphocyte counts were significantly increased for both allergic and non-allergic participants post-exposure. Serum IL-13 concentrations were significantly reduced post-exposure in allergics while TNF-α was significantly reduced in non-allergics. The HDM-EEU is a useful model for investigating biologic mechanisms of HDM-induced AR. Allergic participants produced measurable biological changes compared to healthy controls following allergen exposure, specifically with serum expression of eosinophils and related markers, namely IL-5, which promotes the proliferation and differentiation of eosinophils, and IL-13, a cytokine released by eosinophils. The exact mechanisms at play require further investigation.
变应性鼻炎（AR）是一种鼻黏膜炎症性疾病，可通过可控变应原暴露设施（CACF）进行模拟。最近，我们对屋尘螨（HDM）环境暴露单元（EEU）设施进行了临床验证。在本研究中，我们旨在评估HDM-EEU中HDM暴露后血液中的生物学反应。55名参与者通过了筛选访视，在此过程中他们签署了知情同意书并完成了皮肤点刺试验（SPT），然后参加了适度或更高剂量的HDM暴露环节。采集了基线和暴露后的血液样本。测量了全血细胞计数及分类，并使用分离的血清测定特异性IgE（sIgE）和细胞因子浓度（IL-4、IL-5、IL-6、IL-10、IL-13、TNF-α）。HDM过敏参与者的SPT风团大小明显大于健康对照。过敏参与者的sIgE浓度明显更高，且[此处原文缺失具体物质，无法准确翻译]与[此处原文缺失具体物质，无法准确翻译]之间存在强相关性。过敏参与者暴露后血清嗜酸性粒细胞计数明显降低。过敏和非过敏参与者暴露后白细胞、中性粒细胞和淋巴细胞计数均明显增加。过敏者暴露后血清IL-13浓度明显降低，而非过敏者TNF-α明显降低。HDM-EEU是研究HDM诱导的AR生物学机制的有用模型。与健康对照相比，过敏参与者在变应原暴露后产生了可测量的生物学变化，特别是血清中嗜酸性粒细胞及相关标志物的表达，即促进嗜酸性粒细胞增殖和分化的IL-5，以及嗜酸性粒细胞释放的细胞因子IL-13。其中确切的作用机制有待进一步研究。
