Department of Chemistry, School of Science, Kwansei Gakuin University.
Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba.
Microbes Environ. 2022;37(6). doi: 10.1264/jsme2.ME22006.
Filamentous fungi grow by the elongation of tubular cells called hyphae and form mycelia through repeated hyphal tip growth and branching. Since hyphal growth is closely related to the ability to secrete large amounts of enzymes or invade host cells, a more detailed understanding and the control of its growth are important in fungal biotechnology, ecology, and pathogenesis. Previous studies using fluorescence imaging revealed many of the molecular mechanisms involved in hyphal growth. Raman microspectroscopy and imaging methods are now attracting increasing attention as powerful alternatives due to their high chemical specificity and label-free, non-destructive properties. Spatially resolved information on the relative abundance, structure, and chemical state of multiple intracellular components may be simultaneously obtained. Although Raman studies on filamentous fungi are still limited, this review introduces recent findings from Raman studies on filamentous fungi and discusses their potential use in the future.
丝状真菌通过管状细胞（称为菌丝）的伸长生长，并通过菌丝尖端的反复生长和分支形成菌丝体。由于菌丝生长与大量酶分泌或入侵宿主细胞的能力密切相关，因此更详细地了解和控制其生长对于真菌生物技术、生态学和发病机制非常重要。以前使用荧光成像研究揭示了许多与菌丝生长相关的分子机制。由于拉曼微光谱和成像方法具有高化学特异性和无需标记、非破坏性的特性，因此作为强大的替代品越来越受到关注。可以同时获得关于多个细胞内成分的相对丰度、结构和化学状态的空间分辨信息。尽管丝状真菌的拉曼研究仍然有限，但本综述介绍了丝状真菌拉曼研究的最新发现，并讨论了它们在未来的潜在用途。
