Department of Advanced Science Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Computational Bio Big-Data Open Innovation Laboratory, AIST-Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
J Nat Prod. 2020 Nov 25;83(11):3223-3229. doi: 10.1021/acs.jnatprod.0c00214. Epub 2020 Oct 19.
Raman microspectroscopy is a minimally invasive technique that can identify molecules without labeling. In this study, we demonstrate the detection of penicillin G inside KF425 fungal cells. Raman spectra acquired from the fungal cells had highly overlapped spectroscopic signatures and hence were analyzed with multivariate curve resolution by alternating least-squares (MCR-ALS) to extract the spectra of individual molecular constituents. In addition to detecting spatial distribution of multiple constituents such as proteins and lipids inside the fungal body, we could also observe the subcellular localization of penicillin G. This methodology has the potential to be employed in screening the production of bioactive compounds by microorganisms.
拉曼微光谱技术是一种无需标记即可识别分子的微创技术。在这项研究中，我们展示了青霉素 G 在 KF425 真菌细胞内的检测。从真菌细胞中获得的拉曼光谱具有高度重叠的光谱特征，因此采用交替最小二乘法（MCR-ALS）的多元曲线分辨分析来提取各个分子成分的光谱。除了检测真菌体内多种成分（如蛋白质和脂质）的空间分布外，我们还可以观察青霉素 G 的亚细胞定位。这种方法有可能用于筛选微生物产生的生物活性化合物。
