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使用RIM大肠杆菌检测试剂盒快速鉴定大肠杆菌。

Use of the RIM Escherichia coli Kit for rapid identification of Escherichia coli.

作者信息

Varga F J, DiPersio J R

出版信息

Am J Clin Pathol. 1986 Dec;86(6):761-4. doi: 10.1093/ajcp/86.6.761.

DOI:10.1093/ajcp/86.6.761
PMID:3538847
Abstract

The Rapid Identification Method (RIM) Escherichia coli Kit (Austin Biologicals Laboratories Inc., Austin, TX) was evaluated as a means to rapidly differentiate at reduced cost clinical isolates of Escherichia coli from other members of the Enterobacteriaceae. A group of 408 gram-negative rods that were isolated on primary isolation plates were tested with the use of both the kit and complete bacterial identification. The RIM E. coli Kit identified 92.5% (272 of 294) of the E. coli isolates. Only 1 of 114 non-E. coli isolates was misidentified as E. coli (positive predictive value equal to 99.6). Although the RIM E. coli Kit was sufficiently rapid (less than one hour), any supply cost savings would depend upon the proportion of true E. coli in the group of organisms tested. Savings derived from the lower cost of using the RIM E. coli Kit would be reduced by the extra cost of screening non-E. coli isolates, which would then have to be completely identified.

摘要

快速鉴定方法(RIM)大肠杆菌检测试剂盒(奥斯汀生物实验室公司,得克萨斯州奥斯汀)被评估为一种以较低成本快速区分临床分离的大肠杆菌与肠杆菌科其他成员的方法。使用该试剂盒和完整细菌鉴定方法对一组在初次分离平板上分离出的408株革兰氏阴性杆菌进行了检测。RIM大肠杆菌检测试剂盒鉴定出了92.5%(294株中的272株)的大肠杆菌分离株。114株非大肠杆菌分离株中只有1株被误鉴定为大肠杆菌(阳性预测值等于99.6)。尽管RIM大肠杆菌检测试剂盒足够快速(不到一小时),但任何供应成本的节省都将取决于所检测生物体群体中真正大肠杆菌的比例。使用RIM大肠杆菌检测试剂盒较低成本所带来的节省,会因筛查非大肠杆菌分离株的额外成本而减少,因为这些非大肠杆菌分离株随后必须进行完整鉴定。

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Use of the RIM Escherichia coli Kit for rapid identification of Escherichia coli.使用RIM大肠杆菌检测试剂盒快速鉴定大肠杆菌。
Am J Clin Pathol. 1986 Dec;86(6):761-4. doi: 10.1093/ajcp/86.6.761.
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引用本文的文献

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Comparison of the RIM-H rapid identification kit with conventional tests for the identification of Haemophilus spp.RIM-H快速鉴定试剂盒与传统方法鉴定嗜血杆菌属的比较
J Clin Microbiol. 1990 Aug;28(8):1862-3. doi: 10.1128/jcm.28.8.1862-1863.1990.
2
Fluorogenic and chromogenic substrates used in bacterial diagnostics.用于细菌诊断的荧光和显色底物。
Microbiol Rev. 1991 Sep;55(3):335-48. doi: 10.1128/mr.55.3.335-348.1991.