Labied Soraya, Jouan Caroline, Wenders Frédéric, Ravet Stéphanie, Gaspard Olivier, Thonon Fabienne, Gridelet Virginie, Henry Laurie, Perrier d'Hauterive Sophie, Nisolle Michelle
a Center for Assisted Medical Procreation University of Liège, CHR Citadelle , Liège , Belgium.
b Department of Obstetrics and Gynecology, CHR Citadelle , University of Liège , Liège , Belgium.
Syst Biol Reprod Med. 2019 Feb;65(1):81-86. doi: 10.1080/19396368.2018.1492645. Epub 2018 Jul 9.
The oil overlay in microdrop culture systems prevents medium evaporation, helps to maintain appropriate pH and osmotic conditions and protects from microbial contamination. In the present study, we prospectively compared covering by Ovoil™, a paraffin oil, and LiteOil®, a mineral oil, on the in vitro development of human embryos and their suitability for transfer/freezing at day 3 and live birth rate. One hundred and one patients undergoing in vitro fertilization (IVF) treatment by intracytoplasmic sperm injection (ICSI) were enrolled in our study. After ICSI, 1237 oocytes were 1:1 randomly allocated into 2 groups according to the type of overlaying oil: Ovoil™ (616 oocytes) or LiteOil® (621 oocytes). Fertilization rate was assessed around 18 hours post-insemination (hpi) and embryos were checked for early cleavage at 25 hpi. Embryo morphology was recorded on days 2 and 3. A total of 437 (Ovoil™) and 438 day 3 embryos (LiteOil®) were analyzed. There were no differences between the two groups in terms of fertilization rate and occurrence of early cleavage. The proportion of top quality embryos (41.7% vs. 41.2%) and the final utilization rates (92.2% vs. 92.0%) were similar in Ovoil and LiteOil groups, respectively, at day 3. Live birth rate per transfer was essentially the same with Ovoil™ overlay (26.9%) when compared to LiteOil® (26.2%). Live birth rate in patients who simultaneously received embryos from both overlay types was 17.2%. Despite the different characteristics of these two oils regarding hydrocarbon saturation, packing and temperature storage, Ovoil™ and LiteOil® can be used in parallel in the same IVF protocol. Abbreviations: ART: assisted reproductive technologies; hpi: hours post-insemination; hSA: human serum albumin; HTF: human tubal fluid; ICSI: intracytoplasmic sperm injection; IVF: in vitro fertilization; MII: metaphase II; MEA: mouse embryo assay; RT: room temperature.
微滴培养系统中的油覆盖层可防止培养基蒸发，有助于维持合适的pH值和渗透压条件，并防止微生物污染。在本研究中，我们前瞻性地比较了石蜡油Ovoil™和矿物油LiteOil®对人类胚胎体外发育的影响，以及它们在第3天用于移植/冷冻的适宜性和活产率。101例行体外受精（IVF）治疗并采用卵胞浆内单精子注射（ICSI）的患者纳入我们的研究。ICSI后，1237枚卵母细胞根据覆盖油的类型1:1随机分为2组：Ovoil™组（616枚卵母细胞）或LiteOil®组（621枚卵母细胞）。授精后约18小时（hpi）评估受精率，并在25 hpi检查胚胎的早期分裂情况。在第2天和第3天记录胚胎形态。共分析了437枚（Ovoil™组）和438枚第3天的胚胎（LiteOil®组）。两组在受精率和早期分裂发生率方面无差异。在第3天，Ovoil组和LiteOil组优质胚胎的比例（分别为41.7%和41.2%）和最终利用率（分别为92.2%和92.0%）相似。与LiteOil®覆盖（26.2%）相比，Ovoil™覆盖时每次移植的活产率基本相同（26.9%）。同时接受两种覆盖类型胚胎的患者的活产率为17.2%。尽管这两种油在碳氢化合物饱和度、包装和温度储存方面存在不同特性，但Ovoil™和LiteOil®可在同一IVF方案中并行使用。缩写：ART：辅助生殖技术；hpi：授精后小时数；hSA：人血清白蛋白；HTF：人输卵管液；ICSI：卵胞浆内单精子注射；IVF：体外受精；MII：中期II；MEA：小鼠胚胎试验；RT：室温。
