Institute of Structural and Molecular Biology, University College London, London WC1E 6BT, UK.
Institute of Structural and Molecular Biology, University College London, London WC1E 6BT, UK. Electronic address: https://twitter.com/csburridge.
Curr Opin Struct Biol. 2022 Jun;74:102357. doi: 10.1016/j.sbi.2022.102357. Epub 2022 Apr 4.
Proteins can begin the conformational search for their native structure in parallel with biosynthesis on the ribosome, in a process termed co-translational folding. In contrast to the reversible folding of isolated domains, as a nascent chain emerges from the ribosome exit tunnel during translation the free energy landscape it explores also evolves as a function of chain length. While this presents a substantially more complex measurement problem, this review will outline the progress that has been made recently in understanding, quantitatively, the process by which a nascent chain attains its full native stability, as well as the mechanisms through which interactions with the nearby ribosome surface can perturb or modulate this process.
蛋白质可以在核糖体上进行生物合成的同时开始其天然结构的构象搜索，这个过程称为共翻译折叠。与孤立结构域的可逆折叠不同，当新生链在翻译过程中从核糖体出口隧道中出来时，它所探索的自由能景观也会随着链长的变化而演变。虽然这提出了一个实质性的更复杂的测量问题，但本综述将概述最近在理解新生链获得其完全天然稳定性的过程方面所取得的进展，以及与附近核糖体表面的相互作用如何干扰或调节这个过程的机制。
