Liu Xiaoqin, Wang Ting, Wu Yuwei, Tan Yifu, Jiang Ting, Li Ke, Lou Beibei, Chen Liwei, Liu Yanfei, Liu Zhenbao
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, Hunan Province, PR China.
Department of Pharmaceutics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013, Hunan Province, PR China.
Biosens Bioelectron. 2022 Jul 15;208:114231. doi: 10.1016/j.bios.2022.114231. Epub 2022 Mar 30.
Biosensors have been employed for monitoring and imaging biological events and molecules. Sensitive detection of different biomolecules in vivo can reflect the changes of physiological conditions in real-time, which is of great significance for the diagnosis and treatment of diseases. The detection of intracellular molecules concentration change can indicate the occurrence and development of disease. But the analysis process of the existing detection methods, such as Western blot detection of intracellular protein, polymerase chain reaction (PCR) technique quantitative analysis of intracellular RNA and DNA, usually need to extract the cell lysis which is complex and time-consuming. Fluorescence bioimaging enables in situ monitoring of intracellular molecules in living cells. By combining the specificity of aptamer for intracellular molecules binding, and biocompatibility of fluorescent materials and nanomaterials, biosensors with different nanostructures have been developed to enter into living cells for analysis. This review summarizes the fluorescence detection methods based on aptamer for intracellular molecules detection. The principles, limit of detection, advantages, and disadvantages of different platforms for intracellular molecular fluorescent response are summarized and reviewed. Finally, the current challenges and future developments were discussed and proposed.
生物传感器已被用于监测和成像生物事件及分子。体内不同生物分子的灵敏检测能够实时反映生理状况的变化，这对疾病的诊断和治疗具有重要意义。细胞内分子浓度变化的检测可以指示疾病的发生和发展。但是现有检测方法的分析过程，如蛋白质免疫印迹法检测细胞内蛋白质、聚合酶链反应（PCR）技术定量分析细胞内RNA和DNA，通常需要提取细胞裂解物，这一过程复杂且耗时。荧光生物成像能够对活细胞内的分子进行原位监测。通过结合适体对细胞内分子结合的特异性以及荧光材料和纳米材料的生物相容性，已开发出具有不同纳米结构的生物传感器进入活细胞进行分析。本文综述了基于适体的细胞内分子荧光检测方法。总结并评述了不同细胞内分子荧光响应平台的原理、检测限、优点和缺点。最后，讨论并提出了当前面临的挑战和未来的发展方向。
