Girardi F, Nöhammer G, Pickel H
Basic Appl Histochem. 1986;30(3):375-86.
The total extinction of apparently normal intermediate cell nuclei was microphotometrically measured in formalin-fixed Feulgen-stained paraffin sections of the uterine cervix. We investigated whether reproducible microphotometric results can be obtained from a histologic section expected to contain a certain amount of sectioned nuclei and stained by a standard technique. The results have shown that exact reproduction of microphotometrically measured mean total extinction values of intermediate cell nuclei can be achieved with a threshold value of 90% transmission in 10 microns sections.
在福尔马林固定、Feulgen染色的子宫颈石蜡切片中，用显微光度计测量明显正常的中间细胞核的总消光值。我们研究了能否从预期含有一定数量切片细胞核并采用标准技术染色的组织学切片中获得可重复的显微光度测量结果。结果表明，在10微米厚的切片中，当透光率阈值为90%时，可实现中间细胞核显微光度测量平均总消光值的精确再现。
