Delgado R, Mikuz G, Hofstädter F
Pathol Res Pract. 1984 Sep;179(1):92-4. doi: 10.1016/S0344-0338(84)80067-9.
This study describes a method for the isolation of cells from paraffin embedded tissues for DNA-Feulgen-cytophotometric measurements. The relevance of the method used is demonstrated by the analysis of the DNA-distribution pattern of cells isolated from an alveolar sarcoma of the soft tissues of a 10-year old girl. The comparison between freshly prepared imprint preparations and specimens after pepsin--extraction clearly shows, that the preparation mode used did not influence the relative DNA-content. The mean values of DNA/nuclei did not show a decrease even after prolonged pepsin treatment (90 minutes). Therefore this method permits access to stored histopathological material for retrospective DNA-Feulgen-Cytophotometric investigations.
本研究描述了一种从石蜡包埋组织中分离细胞以进行DNA福尔根细胞光度测量的方法。通过对一名10岁女孩软组织肺泡肉瘤分离出的细胞的DNA分布模式分析，证明了所使用方法的相关性。新鲜制备的压片标本与胃蛋白酶提取后的标本之间的比较清楚地表明，所采用的制备方式不影响相对DNA含量。即使经过长时间的胃蛋白酶处理（90分钟），DNA/细胞核的平均值也没有下降。因此，该方法允许获取储存的组织病理学材料用于回顾性DNA福尔根细胞光度研究。
