• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌环腺苷酸受体蛋白的过量生产及工程化C末端DNA结合结构域的表达。

Overproduction of the cyclic AMP receptor protein of Escherichia coli and expression of the engineered C-terminal DNA-binding domain.

作者信息

Gronenborn A M, Clore G M

出版信息

Biochem J. 1986 Jun 15;236(3):643-9. doi: 10.1042/bj2360643.

DOI:10.1042/bj2360643
PMID:3539103
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1146894/
Abstract

Overproduction of the cyclic AMP receptor protein (CRP) from Escherichia coli, up to 25% of the soluble cell protein, has been achieved in an inducible host-vector system under transcriptional control of the lambda promoter PL. This system is ideally suited for large scale production and purification of CRP. In addition, a structural gene for the DNA-binding domain of CRP has been constructed. To this end the nucleotide sequence coding for the C-terminus was fused to the sequence coding for the first 10 N-terminal amino acids and cloned into suitable vectors. Good expression was achieved using the lambda PL promoter. The gene product, beta CRP, is recognized by anti-CRP antibodies.

摘要

在λ启动子PL的转录控制下，利用诱导型宿主-载体系统已实现从大肠杆菌中过量生产环腺苷酸受体蛋白（CRP），产量高达可溶性细胞蛋白的25%。该系统非常适合大规模生产和纯化CRP。此外，还构建了CRP DNA结合结构域的结构基因。为此，将编码C末端的核苷酸序列与编码前10个N末端氨基酸的序列融合，并克隆到合适的载体中。使用λPL启动子可实现良好的表达。基因产物βCRP可被抗CRP抗体识别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e0/1146894/0e32d49fe1cd/biochemj00277-0031-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e0/1146894/7986d4d49a3a/biochemj00277-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e0/1146894/830e8e18dc58/biochemj00277-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e0/1146894/4f009878a566/biochemj00277-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e0/1146894/0e32d49fe1cd/biochemj00277-0031-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e0/1146894/7986d4d49a3a/biochemj00277-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e0/1146894/830e8e18dc58/biochemj00277-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e0/1146894/4f009878a566/biochemj00277-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e0/1146894/0e32d49fe1cd/biochemj00277-0031-b.jpg

相似文献

1
Overproduction of the cyclic AMP receptor protein of Escherichia coli and expression of the engineered C-terminal DNA-binding domain.大肠杆菌环腺苷酸受体蛋白的过量生产及工程化C末端DNA结合结构域的表达。
Biochem J. 1986 Jun 15;236(3):643-9. doi: 10.1042/bj2360643.
2
Cloning and sequence of the crp gene of Escherichia coli K 12.大肠杆菌K12 crp基因的克隆与序列分析
Nucleic Acids Res. 1982 Feb 25;10(4):1363-78. doi: 10.1093/nar/10.4.1363.
3
Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein.大肠杆菌环磷酸腺苷受体蛋白基因的分子克隆与核苷酸测序
Nucleic Acids Res. 1982 Feb 25;10(4):1345-61. doi: 10.1093/nar/10.4.1345.
4
Mutations in the cyclic AMP binding site of the cyclic AMP receptor protein of Escherichia coli.大肠杆菌环磷酸腺苷受体蛋白环磷酸腺苷结合位点的突变。
Biochem J. 1988 Aug 1;253(3):801-7. doi: 10.1042/bj2530801.
5
Evidence in vivo for autogenous control of the cyclic AMP receptor protein gene (crp) in Escherichia coli by divergent RNA.大肠杆菌中通过反向RNA对环腺苷酸受体蛋白基因（crp）进行自体调控的体内证据。
J Bacteriol. 1988 Nov;170(11):5076-9. doi: 10.1128/jb.170.11.5076-5079.1988.
6
Alterations in the binding site of the cyclic AMP receptor protein at the Escherichia coli galactose operon regulatory region.环腺苷酸受体蛋白在大肠杆菌半乳糖操纵子调控区域结合位点的改变。
Biochem J. 1988 Aug 1;253(3):809-18. doi: 10.1042/bj2530809.
7
Mutations that alter the ability of the Escherichia coli cyclic AMP receptor protein to activate transcription.改变大肠杆菌环磷酸腺苷受体蛋白激活转录能力的突变。
Nucleic Acids Res. 1990 Dec 25;18(24):7243-50. doi: 10.1093/nar/18.24.7243.
8
[Cloning of the gene controlling catabolite repression with the participation of cyclic adenosine monophosphate in Escherichia coli K-12].[大肠杆菌K-12中参与环磷酸腺苷代谢物阻遏调控基因的克隆]
Genetika. 1983 May;19(5):714-9.
9
Antirepression function in Escherichia coli for the cAMP-cAMP receptor protein transcriptional activator.大肠杆菌中cAMP - cAMP受体蛋白转录激活因子的抗阻遏功能。
Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9880-4. doi: 10.1073/pnas.89.20.9880.
10
Mutations in the Escherichia coli operon that define two promoters and the binding site of the cyclic AMP receptor protein.大肠杆菌操纵子中的突变，其定义了两个启动子以及环磷酸腺苷受体蛋白的结合位点。
J Mol Biol. 1982 Jan 15;154(2):211-27. doi: 10.1016/0022-2836(82)90061-4.

引用本文的文献

1
The binding of the cyclic AMP receptor protein to synthetic DNA sites containing permutations in the consensus sequence TGTGA.环磷酸腺苷受体蛋白与包含共有序列TGTGA中排列变化的合成DNA位点的结合。
Biochem J. 1987 Aug 15;246(1):227-32. doi: 10.1042/bj2460227.
2
Subgenes expressing single lipoyl domains of the pyruvate dehydrogenase complex of Escherichia coli.表达大肠杆菌丙酮酸脱氢酶复合体单个硫辛酰结构域的亚基因。
Biochem J. 1987 Aug 1;245(3):869-74. doi: 10.1042/bj2450869.
3
Probing the sequence-specific interaction of the cyclic AMP receptor protein with DNA by site-directed mutagenesis.

本文引用的文献

1
The operator-binding domain of lambda repressor: structure and DNA recognition.λ阻遏蛋白的操纵子结合结构域：结构与DNA识别
Nature. 1982 Jul 29;298(5873):443-7. doi: 10.1038/298443a0.
2
Proposed alpha-helical super-secondary structure associated with protein-dna recognition.与蛋白质-DNA识别相关的拟议α-螺旋超二级结构。
J Mol Biol. 1982 Aug 25;159(4):745-51. doi: 10.1016/0022-2836(82)90111-5.
3
Two helix DNA binding motif of CAP found in lac repressor and gal repressor.在乳糖阻遏物和半乳糖阻遏物中发现的CAP的双螺旋DNA结合基序。
通过定点诱变探究环磷酸腺苷受体蛋白与DNA的序列特异性相互作用。
Biochem J. 1987 Mar 15;242(3):645-53. doi: 10.1042/bj2420645.
Nucleic Acids Res. 1982 Aug 25;10(16):5085-102. doi: 10.1093/nar/10.16.5085.
4
Homology among DNA-binding proteins suggests use of a conserved super-secondary structure.DNA结合蛋白之间的同源性表明存在保守的超二级结构。
Nature. 1982 Jul 29;298(5873):447-51. doi: 10.1038/298447a0.
5
Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding.通过定点诱变重新设计酶结构：酪氨酰tRNA合成酶与ATP结合
Nature. 1982 Oct 21;299(5885):756-8. doi: 10.1038/299756a0.
6
Interproton distance measurements in solution for a double-stranded DNA undecamer comprising a portion of the specific target site for the cycliC AMP receptor protein in the gal operon. A nuclear Overhauser enhancement study.对包含gal操纵子中环状AMP受体蛋白特定靶位点一部分的双链DNA十一聚体在溶液中的质子间距离进行测量。一项核Overhauser增强研究。
FEBS Lett. 1984 Sep 17;175(1):117-23. doi: 10.1016/0014-5793(84)80582-7.
7
Structure of the cro repressor from bacteriophage lambda and its interaction with DNA.来自噬菌体λ的cro阻遏蛋白的结构及其与DNA的相互作用。
Nature. 1981 Apr 30;290(5809):754-8. doi: 10.1038/290754a0.
8
Probing the catalytic mechanism of yeast triose phosphate isomerase by site-specific mutagenesis.通过定点诱变探究酵母磷酸丙糖异构酶的催化机制
Biochem Soc Trans. 1984 Apr;12(2):229-32. doi: 10.1042/bst0120229.
9
Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.携带用于在大肠杆菌中调控克隆基因表达的杂合色氨酸-乳糖启动子的载体。
Gene. 1983 Nov;25(2-3):167-78. doi: 10.1016/0378-1119(83)90222-6.
10
Directed mutagenesis of dihydrofolate reductase.二氢叶酸还原酶的定向诱变
Science. 1983 Nov 18;222(4625):782-8. doi: 10.1126/science.6356360.